Oxidation of 1,2,4,5-tetramethoxybenzene by lignin peroxidase of Phanerochaete chrysosporium

Rao S. Koduri; Ross E. Whitwam; David Barr; Steven D. Aust; Ming Tien

doi:10.1006/abbi.1996.0074

Oxidation of 1,2,4,5-tetramethoxybenzene by lignin peroxidase of Phanerochaete chrysosporium

Rao S. Koduri
, Ross E. Whitwam
, David Barr
, Steven D. Aust
, Ming Tien

Plant Institute

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

We have reinvestigated the lignin peroxidase-catalyzed oxidation of 1,2,4,5-tetramethoxybenzene (TMB) by using presteady-state and steady-state kinetic methods. Our presteady-state kinetic results show that the reaction of compound I with TMB obeyed second order kinetics with a rate constant of 1.1 x 10⁷ M^-1 s^-1. The reaction of compound II with TMB exhibits a hyperbolic concentration dependence with a K(d) of 16 μM and k = 24 s^-1. The stoichiometry of TMB oxidation during steady state is two TMB cation radicals formed per H₂O₂ consumed. These results clearly show that TMB is a good substrate for both compounds I and II of lignin peroxidase.

Original language	English (US)
Pages (from-to)	261-265
Number of pages	5
Journal	Archives of Biochemistry and Biophysics
Volume	326
Issue number	2
DOIs	https://doi.org/10.1006/abbi.1996.0074
State	Published - Feb 15 1996

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology

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10.1006/abbi.1996.0074

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title = "Oxidation of 1,2,4,5-tetramethoxybenzene by lignin peroxidase of Phanerochaete chrysosporium",

abstract = "We have reinvestigated the lignin peroxidase-catalyzed oxidation of 1,2,4,5-tetramethoxybenzene (TMB) by using presteady-state and steady-state kinetic methods. Our presteady-state kinetic results show that the reaction of compound I with TMB obeyed second order kinetics with a rate constant of 1.1 x 107 M-1 s-1. The reaction of compound II with TMB exhibits a hyperbolic concentration dependence with a K(d) of 16 μM and k = 24 s-1. The stoichiometry of TMB oxidation during steady state is two TMB cation radicals formed per H2O2 consumed. These results clearly show that TMB is a good substrate for both compounds I and II of lignin peroxidase.",

author = "Koduri, \{Rao S.\} and Whitwam, \{Ross E.\} and David Barr and Aust, \{Steven D.\} and Ming Tien",

note = "Funding Information: This work was supported in part by Department of Energy Grant DE-FG02-87ER13690 and National Institute of Environmental Health Sciences Grant 1-P42ES04922-01.",

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doi = "10.1006/abbi.1996.0074",

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T1 - Oxidation of 1,2,4,5-tetramethoxybenzene by lignin peroxidase of Phanerochaete chrysosporium

AU - Koduri, Rao S.

AU - Whitwam, Ross E.

AU - Barr, David

AU - Aust, Steven D.

AU - Tien, Ming

N1 - Funding Information: This work was supported in part by Department of Energy Grant DE-FG02-87ER13690 and National Institute of Environmental Health Sciences Grant 1-P42ES04922-01.

PY - 1996/2/15

Y1 - 1996/2/15

N2 - We have reinvestigated the lignin peroxidase-catalyzed oxidation of 1,2,4,5-tetramethoxybenzene (TMB) by using presteady-state and steady-state kinetic methods. Our presteady-state kinetic results show that the reaction of compound I with TMB obeyed second order kinetics with a rate constant of 1.1 x 107 M-1 s-1. The reaction of compound II with TMB exhibits a hyperbolic concentration dependence with a K(d) of 16 μM and k = 24 s-1. The stoichiometry of TMB oxidation during steady state is two TMB cation radicals formed per H2O2 consumed. These results clearly show that TMB is a good substrate for both compounds I and II of lignin peroxidase.

AB - We have reinvestigated the lignin peroxidase-catalyzed oxidation of 1,2,4,5-tetramethoxybenzene (TMB) by using presteady-state and steady-state kinetic methods. Our presteady-state kinetic results show that the reaction of compound I with TMB obeyed second order kinetics with a rate constant of 1.1 x 107 M-1 s-1. The reaction of compound II with TMB exhibits a hyperbolic concentration dependence with a K(d) of 16 μM and k = 24 s-1. The stoichiometry of TMB oxidation during steady state is two TMB cation radicals formed per H2O2 consumed. These results clearly show that TMB is a good substrate for both compounds I and II of lignin peroxidase.

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DO - 10.1006/abbi.1996.0074

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JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

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ER -

Oxidation of 1,2,4,5-tetramethoxybenzene by lignin peroxidase of Phanerochaete chrysosporium

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