Oxidative stress suppression in: C. elegans by peptides from dogfish skin via regulation of transcription factors DAF-16 and HSF-1

Xiaoli Ma, Jiao Li, Yi Zhang, Orcun Hacariz, Jianguo Xia, Benjamin K. Simpson, Zhuanhua Wang

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Functional peptides were obtained via enzymatic hydrolysis of smooth dogfish (Mustelus canis) skin. The enzyme-assisted process was optimized to achieve high yield of smooth dogfish skin peptides (SDSP). Fractions of SDSP (MW < 2 kDa, 2-5 kDa, 5-10 kDa and >10 kDa) showed in vitro antioxidant activities. The peptides <2 kDa (SDSP<2 kDa) significantly improved motility, reduced ROS and H2O2 levels of Caenorhabditis elegans, and increased its resistance to oxidative stress compared to the other peptide fractions. In vivo function of SDSP<2 kDa could be explained by their capacity to increase the expression of stress-response genes. The enhanced resistance to oxidative stress mediated by SDSP<2 kDa was dependent on DAF-16 and HSF-1. The amino acid residues and sequences of SDSP<2 kDa were characterized and revealed a higher content of hydrophobic versus polar amino acid contents. This study (especially the in vivo investigation) explored new potent antioxidant peptides derived from dogfish skin.

Original languageEnglish (US)
Pages (from-to)716-724
Number of pages9
JournalFood and Function
Volume13
Issue number2
DOIs
StatePublished - Jan 21 2022

All Science Journal Classification (ASJC) codes

  • Food Science

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