p120-catenin binding masks an endocytic signal conserved in classical cadherins

Benjamin A. Nanes, Christine Chiasson-MacKenzie, Anthony M. Lowery, Noboru Ishiyama, Victor Faundez, Mitsuhiko Ikura, Peter A. Vincent, Andrew P. Kowalczyk

Research output: Contribution to journalArticlepeer-review

148 Scopus citations


p120-catenin (p120) binds to the cytoplasmic tails of classical cadherins and inhibits cadherin endocytosis. Although p120 regulation of cadherin internalization is thought to be important for adhesive junction dynamics, the mechanism by which p120 modulates cadherin endocytosis is unknown. In this paper, we identify a dual-function motif in classical cadherins consisting of three highly conserved acidic residues that alternately serve as a p120-binding interface and an endocytic signal. Mutation of this motif resulted in a cadherin variant that was both p120 uncoupled and resistant to endocytosis. In endothelial cells, in which dynamic changes in adhesion are important components of angiogenesis and inflammation, a vascular endothelial cadherin (VE-cadherin) mutant defective in endocytosis assembled normally into cell-cell junctions but potently suppressed cell migration in response to vascular endothelial growth factor. These results reveal the mechanistic basis by which p120 stabilizes cadherins and demonstrate that VE-cadherin endocytosis is crucial for endothelial cell migration in response to an angiogenic growth factor.

Original languageEnglish (US)
Pages (from-to)365-380
Number of pages16
JournalJournal of Cell Biology
Issue number2
StatePublished - Oct 15 2012

All Science Journal Classification (ASJC) codes

  • Cell Biology


Dive into the research topics of 'p120-catenin binding masks an endocytic signal conserved in classical cadherins'. Together they form a unique fingerprint.

Cite this