PAb 2000 specifically recognizes the large T and small t proteins of JC virus

Brigitte Bollag; Richard J. Frisque

doi:10.1016/0168-1702(92)90136-W

PAb 2000 specifically recognizes the large T and small t proteins of JC virus

Brigitte Bollag, Richard J. Frisque

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

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Abstract

A monoclonal antibody, PAb 2000, has been derived which recognizes the large T protein of JC virus (JCV), but not the corresponding proteins of the related polyomaviruses BK virus (BKV) and SV40. The epitope bound by PAb 2000 was localized to the amino-terminal 81 amino acids of this multifunctional protein. As observed previously with several monoclonal antibodies that bind a similar region of SV40 large T antigen, PAb 2000 was found to interact with the small t antigen and the denatured form of large T antigen. This monoclonal antibody recognized a subpopulation of T protein, the abundance of which varied in different species of cells transformed by JCV. The availability of PAb 2000, the first JCV T antigenspecific monoclonal antibody, will facilitate the purification and biochemical characterization of the JCV oncoproteins.

Original language	English (US)
Pages (from-to)	223-239
Number of pages	17
Journal	Virus Research
Volume	25
Issue number	3
DOIs	https://doi.org/10.1016/0168-1702(92)90136-W
State	Published - Sep 15 1992

All Science Journal Classification (ASJC) codes

Cancer Research
Virology
Infectious Diseases

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10.1016/0168-1702(92)90136-W

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title = "PAb 2000 specifically recognizes the large T and small t proteins of JC virus",

abstract = "A monoclonal antibody, PAb 2000, has been derived which recognizes the large T protein of JC virus (JCV), but not the corresponding proteins of the related polyomaviruses BK virus (BKV) and SV40. The epitope bound by PAb 2000 was localized to the amino-terminal 81 amino acids of this multifunctional protein. As observed previously with several monoclonal antibodies that bind a similar region of SV40 large T antigen, PAb 2000 was found to interact with the small t antigen and the denatured form of large T antigen. This monoclonal antibody recognized a subpopulation of T protein, the abundance of which varied in different species of cells transformed by JCV. The availability of PAb 2000, the first JCV T antigenspecific monoclonal antibody, will facilitate the purification and biochemical characterization of the JCV oncoproteins.",

author = "Brigitte Bollag and Frisque, {Richard J.}",

note = "Funding Information: We are indebted to A. Karu who, through detailed instruction, taught us hybridomat echnology.W e wish to thank M.J. and S. Tevethia for providingM EF and B6/SRMMT Cl-B cells and PAb 901. We are grateful to G. Lettiery and J. Tavis for helpful discussionsa nd to P. MacKeen for assistancein initiating this project. This work was supportedb y Public Health ServiceG rant CA-38789f rom the National Cancer Institute and by a Biomedical ResearchS upport Grant from the U.S. Department of Health and Human Services, National Institutes of Health.",

year = "1992",

month = sep,

day = "15",

doi = "10.1016/0168-1702(92)90136-W",

language = "English (US)",

volume = "25",

pages = "223--239",

journal = "Virus Research",

issn = "0168-1702",

publisher = "Elsevier B.V.",

number = "3",

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TY - JOUR

T1 - PAb 2000 specifically recognizes the large T and small t proteins of JC virus

AU - Bollag, Brigitte

AU - Frisque, Richard J.

N1 - Funding Information: We are indebted to A. Karu who, through detailed instruction, taught us hybridomat echnology.W e wish to thank M.J. and S. Tevethia for providingM EF and B6/SRMMT Cl-B cells and PAb 901. We are grateful to G. Lettiery and J. Tavis for helpful discussionsa nd to P. MacKeen for assistancein initiating this project. This work was supportedb y Public Health ServiceG rant CA-38789f rom the National Cancer Institute and by a Biomedical ResearchS upport Grant from the U.S. Department of Health and Human Services, National Institutes of Health.

PY - 1992/9/15

Y1 - 1992/9/15

N2 - A monoclonal antibody, PAb 2000, has been derived which recognizes the large T protein of JC virus (JCV), but not the corresponding proteins of the related polyomaviruses BK virus (BKV) and SV40. The epitope bound by PAb 2000 was localized to the amino-terminal 81 amino acids of this multifunctional protein. As observed previously with several monoclonal antibodies that bind a similar region of SV40 large T antigen, PAb 2000 was found to interact with the small t antigen and the denatured form of large T antigen. This monoclonal antibody recognized a subpopulation of T protein, the abundance of which varied in different species of cells transformed by JCV. The availability of PAb 2000, the first JCV T antigenspecific monoclonal antibody, will facilitate the purification and biochemical characterization of the JCV oncoproteins.

AB - A monoclonal antibody, PAb 2000, has been derived which recognizes the large T protein of JC virus (JCV), but not the corresponding proteins of the related polyomaviruses BK virus (BKV) and SV40. The epitope bound by PAb 2000 was localized to the amino-terminal 81 amino acids of this multifunctional protein. As observed previously with several monoclonal antibodies that bind a similar region of SV40 large T antigen, PAb 2000 was found to interact with the small t antigen and the denatured form of large T antigen. This monoclonal antibody recognized a subpopulation of T protein, the abundance of which varied in different species of cells transformed by JCV. The availability of PAb 2000, the first JCV T antigenspecific monoclonal antibody, will facilitate the purification and biochemical characterization of the JCV oncoproteins.

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SN - 0168-1702

VL - 25

SP - 223

EP - 239

JO - Virus Research

JF - Virus Research

IS - 3

ER -

PAb 2000 specifically recognizes the large T and small t proteins of JC virus

Abstract

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