TY - JOUR
T1 - Peptide hormones CHAL, CLL1, and CLL2 are important for stamen development in Arabidopsis
AU - He, Zhuo Na
AU - Wang, Shuang Shuang
AU - Ma, Hong
AU - Chang, Fang
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant No. 31130006).
Publisher Copyright:
© 2016, Science Press. All right reserved.
PY - 2016/2/20
Y1 - 2016/2/20
N2 - Cell-cell communication is important for normal anther development and male fertility, but little is known about signals important for this process. The polypeptide hormones CHAL/CLL1/CLL2 are redundantly required for normal stomata cell development in Arabidopsis, but the chal cll1 cll2 triple mutants exhibited reduced fertility. However, the function of the CHAL/CLL1/CLL2 genes in the regulation of Arabidopsis fertility has not been investigated. In this study, we showed that the CHAL/CLL1/CLL2 genes were highly expressed in anther filaments, tapetal cells, and male reproductive cells at anther stage 5-9. Single mutants of the CHAL/ CLL1/CLL2 genes did not show obvious fertility defects, but the double (chal cll1 and chal cll2) and triple mutants (chal cll1 cll2) showed incremental defects in fertility: short filaments, abnormal tapetal development, aborted pollens, and extremely low pollen germination rate. Further studies showed that, the expression of many anther development-required genes were obviously altered in the chal cll1 cll2 triple mutant, including BAM1, EMS1, TPD1, bHLH089, TSM1, MS1, and AMS. These data together strongly support the idea that CHAL/CLL1/CLL2 play important roles in Arabidopsis stamen development.
AB - Cell-cell communication is important for normal anther development and male fertility, but little is known about signals important for this process. The polypeptide hormones CHAL/CLL1/CLL2 are redundantly required for normal stomata cell development in Arabidopsis, but the chal cll1 cll2 triple mutants exhibited reduced fertility. However, the function of the CHAL/CLL1/CLL2 genes in the regulation of Arabidopsis fertility has not been investigated. In this study, we showed that the CHAL/CLL1/CLL2 genes were highly expressed in anther filaments, tapetal cells, and male reproductive cells at anther stage 5-9. Single mutants of the CHAL/ CLL1/CLL2 genes did not show obvious fertility defects, but the double (chal cll1 and chal cll2) and triple mutants (chal cll1 cll2) showed incremental defects in fertility: short filaments, abnormal tapetal development, aborted pollens, and extremely low pollen germination rate. Further studies showed that, the expression of many anther development-required genes were obviously altered in the chal cll1 cll2 triple mutant, including BAM1, EMS1, TPD1, bHLH089, TSM1, MS1, and AMS. These data together strongly support the idea that CHAL/CLL1/CLL2 play important roles in Arabidopsis stamen development.
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U2 - 10.13592/j.cnki.ppj.2015.0673
DO - 10.13592/j.cnki.ppj.2015.0673
M3 - Article
AN - SCOPUS:85013069658
SN - 2095-1108
VL - 52
SP - 167
EP - 176
JO - Zhiwu Shengli Xuebao/Plant Physiology Journal
JF - Zhiwu Shengli Xuebao/Plant Physiology Journal
IS - 2
ER -