TY - JOUR
T1 - Persistent reduced oxygen requirement following blood transfusion during recovery from hemorrhagic shock
AU - Haouzi, Philippe
AU - Van de Louw, Andry
N1 - Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/8/5
Y1 - 2015/8/5
N2 - Our study intended to determine the effects on oxygen uptake (V ˙O2) of restoring a normal rate of O2 delivery following blood transfusion (BT) after a severe hemorrhage (H). Spontaneously breathing urethane anesthetized rats were bled by removing 20ml/kg of blood over 30min. Rats were then infused with their own shed blood 15min after the end of H. At mid-perfusion, half of the rats received a unique infusion of the decoupling agent 2,4-dinitrophenol (DNP, 6mg/kg). V ˙O2 and arterial blood pressure (ABP) were continuously measured throughout the study, along with serial determination of blood lactate concentration [La]. Animals were euthanized 45min after the end of reperfusion; liver and lungs were further analyzed for early expression of oxidative stress gene using RT-PCR. Our bleeding protocol induced a significant decrease in ABP and increase in [La], while V ˙O2 dropped by half. The O2 deficit progressively accumulated during the period of bleeding reached -114±53ml/kg, just before blood transfusion. Despite the transfusion of blood, a significant O2 deficit persisted (-82±59ml/kg) 45min after reperfusion. This slow recovery of V ˙O2 was sped up by DNP injection, leading to a fast recovery of O2 deficit after reperfusion, becoming positive (+460±132ml/kg) by the end of the protocol, supporting the view that O2 supply is not the main controller of V ˙O2 dynamics after BT. Of note is that DNP also enhanced oxidative stress gene expression (up-regulation of NADPH oxidase 4 in the lung for instance). The mechanism of slow recovery of O2 requirement/demand following BT and the resulting effects on tissues exposed to relatively high O2 partial pressure are discussed.
AB - Our study intended to determine the effects on oxygen uptake (V ˙O2) of restoring a normal rate of O2 delivery following blood transfusion (BT) after a severe hemorrhage (H). Spontaneously breathing urethane anesthetized rats were bled by removing 20ml/kg of blood over 30min. Rats were then infused with their own shed blood 15min after the end of H. At mid-perfusion, half of the rats received a unique infusion of the decoupling agent 2,4-dinitrophenol (DNP, 6mg/kg). V ˙O2 and arterial blood pressure (ABP) were continuously measured throughout the study, along with serial determination of blood lactate concentration [La]. Animals were euthanized 45min after the end of reperfusion; liver and lungs were further analyzed for early expression of oxidative stress gene using RT-PCR. Our bleeding protocol induced a significant decrease in ABP and increase in [La], while V ˙O2 dropped by half. The O2 deficit progressively accumulated during the period of bleeding reached -114±53ml/kg, just before blood transfusion. Despite the transfusion of blood, a significant O2 deficit persisted (-82±59ml/kg) 45min after reperfusion. This slow recovery of V ˙O2 was sped up by DNP injection, leading to a fast recovery of O2 deficit after reperfusion, becoming positive (+460±132ml/kg) by the end of the protocol, supporting the view that O2 supply is not the main controller of V ˙O2 dynamics after BT. Of note is that DNP also enhanced oxidative stress gene expression (up-regulation of NADPH oxidase 4 in the lung for instance). The mechanism of slow recovery of O2 requirement/demand following BT and the resulting effects on tissues exposed to relatively high O2 partial pressure are discussed.
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U2 - 10.1016/j.resp.2015.04.009
DO - 10.1016/j.resp.2015.04.009
M3 - Article
C2 - 25911557
AN - SCOPUS:84929379075
SN - 1569-9048
VL - 215
SP - 39
EP - 46
JO - Respiratory Physiology and Neurobiology
JF - Respiratory Physiology and Neurobiology
ER -