TY - JOUR
T1 - Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation
AU - Lerch-Gaggl, Alexandra
AU - Haque, Jamil
AU - Li, Jixuan
AU - Ning, Gang
AU - Traktman, Paula
AU - Duncan, Stephen A.
PY - 2002/11/22
Y1 - 2002/11/22
N2 - Mutation of the zebrafish pescadillo gene blocks expansion of a number of tissues in the developing embryo, suggesting roles for its gene product in controlling cell proliferation. We report that levels of the pescadillo protein increase in rodent hepatocytes as they enter the cell cycle. Pescadillo protein localizes to distinct substructures of the interphase nucleus including nucleoli, the site of ribosome biogenesis. During mitosis pescadillo closely associates with the periphery of metaphase chromosomes and by late anaphase is associated with nucleolus-derived foci and prenucleolar bodies. Blastomeres in mouse embryos lacking pescadillo arrest at morula stages of development, the nucleoli fail to differentiate and accumulation of ribosomes is inhibited. We propose that in mammalian cells pescadillo is essential for ribosome biogenesis and nucleologenesis and that disruption to its function results in cell cycle arrest.
AB - Mutation of the zebrafish pescadillo gene blocks expansion of a number of tissues in the developing embryo, suggesting roles for its gene product in controlling cell proliferation. We report that levels of the pescadillo protein increase in rodent hepatocytes as they enter the cell cycle. Pescadillo protein localizes to distinct substructures of the interphase nucleus including nucleoli, the site of ribosome biogenesis. During mitosis pescadillo closely associates with the periphery of metaphase chromosomes and by late anaphase is associated with nucleolus-derived foci and prenucleolar bodies. Blastomeres in mouse embryos lacking pescadillo arrest at morula stages of development, the nucleoli fail to differentiate and accumulation of ribosomes is inhibited. We propose that in mammalian cells pescadillo is essential for ribosome biogenesis and nucleologenesis and that disruption to its function results in cell cycle arrest.
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U2 - 10.1074/jbc.M208338200
DO - 10.1074/jbc.M208338200
M3 - Article
C2 - 12237316
AN - SCOPUS:0037160082
SN - 0021-9258
VL - 277
SP - 45347
EP - 45355
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 47
ER -