Phase contrast imaging is performed for live biological species using photothermal induced birefringence in dye doped liquid crystals. Using typical 4-f configuration, when liquid crystal cell is at back focal plane of Fourier lens, low spatial frequencies at center of Fourier spectrum are intense enough to induce local liquid crystal molecules into isotropic phase, whereas high spatial frequencies on the edges are not intense enough and remain in anisotropic phase. This results in π 2 phase difference between high and low spatial frequencies. This simple, inexpensive, all-optical, user-friendly, self-adaptive phase contrast imaging technique using low-power laser offers several distinct advantages.
All Science Journal Classification (ASJC) codes
- Physics and Astronomy (miscellaneous)