Phenotypic expressions of CCR5-Δ32/Δ32 homozygosity

Giang T. Nguyêñ; Mary Carrington; Judith A. Beeler; Michael Dean; Louis M. Aledort; Philip M. Blatt; Alan R. Cohen; Donna DiMichele; M. Elaine Eyster; Craig M. Kessler; Barbara Konkle; Cindy Leissinger; Naomi Luban; Stephen J. O'Brien; James J. Goedert; Thomas R. O'Brien

doi:10.1097/00042560-199909010-00010

Phenotypic expressions of CCR5-Δ32/Δ32 homozygosity

Giang T. Nguyêñ, Mary Carrington, Judith A. Beeler, Michael Dean, Louis M. Aledort, Philip M. Blatt, Alan R. Cohen, Donna DiMichele, M. Elaine Eyster, Craig M. Kessler, Barbara Konkle, Cindy Leissinger, Naomi Luban, Stephen J. O'Brien, James J. Goedert, Thomas R. O'Brien

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Abstract

Objective: As blockade of CC-chemokine receptor 5 (CCR5) has been proposed as therapy for HIV-1, we examined whether the CCR5-Δ32/Δ32 homozygous genotype has phenotypic expressions other than those related to HIV-1. Design: Study subjects were white homosexual men or men with hemophilia who were not infected with HIV-1. In this study, 15 CCR5-Δ32/Δ32 homozygotes were compared with 201 CCR5 wild-type (+/+) subjects for a wide range of clinical conditions and laboratory assay results ascertained during prospective cohort studies and routine clinical care. CCR5-Δ32 genotype was determined by polymerase chain reaction, followed by single-stranded conformational polymorphism analysis. Results: Hypertension and conditions attributable to hemophilia were the only diagnoses frequently found in clinical records of CCR5-Δ32/Δ32 study subjects. Based on blood pressure measurement and treatment history, CCR5-Δ32/Δ32 homozygotes had a 2.8-fold higher prevalence of hypertension than age-matched CCR5-+/+ study subjects (95% confidence interval [CI], 1.2-6.4; p = .01); none of the homozygotes had severe hypertension. Hematologic measures were generally similar across the genotypes, but total lymphocyte counts were ~20% higher in CCR5-Δ32/Δ32 study subjects than in CCR5-+/+ study subjects (p < .05). Among patients with hemophilia who were infected with hepatitis C virus (HCV), mean alanine aminotransferase levels were 117% higher among CCR5-Δ32/Δ32 homozygotes (p < .05), but serum HCV levels did not differ by CCR5-Δ32 genotype. CCR5- Δ32/Δ32 homozygous study subjects had a lower prevalence of antibodies to measles virus than those with other genotypes, but this association was not confirmed in a group of blood donors. The prevalence of antibodies to nine other common viruses, HBV, and HCV was not related to CCR5 genotype. Conclusions: CCR5-Δ32/Δ32 homozygotes are generally similar to wild-type persons. Confirmatory investigations are required to determine whether hypertension, increased lymphocyte counts, and higher hepatic enzyme levels in the presence of HCV infection represent true phenotypic expressions of this genotype. CCR5-Δ32/Δ32 homozygosity does not provide broad protection against viral infections.

Original language	English (US)
Pages (from-to)	75-82
Number of pages	8
Journal	Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume	22
Issue number	1
DOIs	https://doi.org/10.1097/00042560-199909010-00010
State	Published - Sep 1 1999

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Virology

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10.1097/00042560-199909010-00010

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Nguyêñ, G. T., Carrington, M., Beeler, J. A., Dean, M., Aledort, L. M., Blatt, P. M., Cohen, A. R., DiMichele, D., Eyster, M. E., Kessler, C. M., Konkle, B., Leissinger, C., Luban, N., O'Brien, S. J., Goedert, J. J., & O'Brien, T. R. (1999). Phenotypic expressions of CCR5-Δ32/Δ32 homozygosity. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, 22(1), 75-82. https://doi.org/10.1097/00042560-199909010-00010

@article{5237591eb53d466ebd76bcdd9e9d9890,

title = "Phenotypic expressions of CCR5-Δ32/Δ32 homozygosity",

abstract = "Objective: As blockade of CC-chemokine receptor 5 (CCR5) has been proposed as therapy for HIV-1, we examined whether the CCR5-Δ32/Δ32 homozygous genotype has phenotypic expressions other than those related to HIV-1. Design: Study subjects were white homosexual men or men with hemophilia who were not infected with HIV-1. In this study, 15 CCR5-Δ32/Δ32 homozygotes were compared with 201 CCR5 wild-type (+/+) subjects for a wide range of clinical conditions and laboratory assay results ascertained during prospective cohort studies and routine clinical care. CCR5-Δ32 genotype was determined by polymerase chain reaction, followed by single-stranded conformational polymorphism analysis. Results: Hypertension and conditions attributable to hemophilia were the only diagnoses frequently found in clinical records of CCR5-Δ32/Δ32 study subjects. Based on blood pressure measurement and treatment history, CCR5-Δ32/Δ32 homozygotes had a 2.8-fold higher prevalence of hypertension than age-matched CCR5-+/+ study subjects (95% confidence interval [CI], 1.2-6.4; p = .01); none of the homozygotes had severe hypertension. Hematologic measures were generally similar across the genotypes, but total lymphocyte counts were ~20% higher in CCR5-Δ32/Δ32 study subjects than in CCR5-+/+ study subjects (p < .05). Among patients with hemophilia who were infected with hepatitis C virus (HCV), mean alanine aminotransferase levels were 117% higher among CCR5-Δ32/Δ32 homozygotes (p < .05), but serum HCV levels did not differ by CCR5-Δ32 genotype. CCR5- Δ32/Δ32 homozygous study subjects had a lower prevalence of antibodies to measles virus than those with other genotypes, but this association was not confirmed in a group of blood donors. The prevalence of antibodies to nine other common viruses, HBV, and HCV was not related to CCR5 genotype. Conclusions: CCR5-Δ32/Δ32 homozygotes are generally similar to wild-type persons. Confirmatory investigations are required to determine whether hypertension, increased lymphocyte counts, and higher hepatic enzyme levels in the presence of HCV infection represent true phenotypic expressions of this genotype. CCR5-Δ32/Δ32 homozygosity does not provide broad protection against viral infections.",

author = "Nguy{\^e}{\~n}, {Giang T.} and Mary Carrington and Beeler, {Judith A.} and Michael Dean and Aledort, {Louis M.} and Blatt, {Philip M.} and Cohen, {Alan R.} and Donna DiMichele and Eyster, {M. Elaine} and Kessler, {Craig M.} and Barbara Konkle and Cindy Leissinger and Naomi Luban and O'Brien, {Stephen J.} and Goedert, {James J.} and O'Brien, {Thomas R.}",

year = "1999",

month = sep,

day = "1",

doi = "10.1097/00042560-199909010-00010",

language = "English (US)",

volume = "22",

pages = "75--82",

journal = "Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology",

issn = "1077-9450",

publisher = "Wolters Kluwer Health",

number = "1",

}

Nguyêñ, GT, Carrington, M, Beeler, JA, Dean, M, Aledort, LM, Blatt, PM, Cohen, AR, DiMichele, D, Eyster, ME, Kessler, CM, Konkle, B, Leissinger, C, Luban, N, O'Brien, SJ, Goedert, JJ & O'Brien, TR 1999, 'Phenotypic expressions of CCR5-Δ32/Δ32 homozygosity', Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, vol. 22, no. 1, pp. 75-82. https://doi.org/10.1097/00042560-199909010-00010

TY - JOUR

T1 - Phenotypic expressions of CCR5-Δ32/Δ32 homozygosity

AU - Nguyêñ, Giang T.

AU - Carrington, Mary

AU - Beeler, Judith A.

AU - Dean, Michael

AU - Aledort, Louis M.

AU - Blatt, Philip M.

AU - Cohen, Alan R.

AU - DiMichele, Donna

AU - Eyster, M. Elaine

AU - Kessler, Craig M.

AU - Konkle, Barbara

AU - Leissinger, Cindy

AU - Luban, Naomi

AU - O'Brien, Stephen J.

AU - Goedert, James J.

AU - O'Brien, Thomas R.

PY - 1999/9/1

Y1 - 1999/9/1

N2 - Objective: As blockade of CC-chemokine receptor 5 (CCR5) has been proposed as therapy for HIV-1, we examined whether the CCR5-Δ32/Δ32 homozygous genotype has phenotypic expressions other than those related to HIV-1. Design: Study subjects were white homosexual men or men with hemophilia who were not infected with HIV-1. In this study, 15 CCR5-Δ32/Δ32 homozygotes were compared with 201 CCR5 wild-type (+/+) subjects for a wide range of clinical conditions and laboratory assay results ascertained during prospective cohort studies and routine clinical care. CCR5-Δ32 genotype was determined by polymerase chain reaction, followed by single-stranded conformational polymorphism analysis. Results: Hypertension and conditions attributable to hemophilia were the only diagnoses frequently found in clinical records of CCR5-Δ32/Δ32 study subjects. Based on blood pressure measurement and treatment history, CCR5-Δ32/Δ32 homozygotes had a 2.8-fold higher prevalence of hypertension than age-matched CCR5-+/+ study subjects (95% confidence interval [CI], 1.2-6.4; p = .01); none of the homozygotes had severe hypertension. Hematologic measures were generally similar across the genotypes, but total lymphocyte counts were ~20% higher in CCR5-Δ32/Δ32 study subjects than in CCR5-+/+ study subjects (p < .05). Among patients with hemophilia who were infected with hepatitis C virus (HCV), mean alanine aminotransferase levels were 117% higher among CCR5-Δ32/Δ32 homozygotes (p < .05), but serum HCV levels did not differ by CCR5-Δ32 genotype. CCR5- Δ32/Δ32 homozygous study subjects had a lower prevalence of antibodies to measles virus than those with other genotypes, but this association was not confirmed in a group of blood donors. The prevalence of antibodies to nine other common viruses, HBV, and HCV was not related to CCR5 genotype. Conclusions: CCR5-Δ32/Δ32 homozygotes are generally similar to wild-type persons. Confirmatory investigations are required to determine whether hypertension, increased lymphocyte counts, and higher hepatic enzyme levels in the presence of HCV infection represent true phenotypic expressions of this genotype. CCR5-Δ32/Δ32 homozygosity does not provide broad protection against viral infections.

AB - Objective: As blockade of CC-chemokine receptor 5 (CCR5) has been proposed as therapy for HIV-1, we examined whether the CCR5-Δ32/Δ32 homozygous genotype has phenotypic expressions other than those related to HIV-1. Design: Study subjects were white homosexual men or men with hemophilia who were not infected with HIV-1. In this study, 15 CCR5-Δ32/Δ32 homozygotes were compared with 201 CCR5 wild-type (+/+) subjects for a wide range of clinical conditions and laboratory assay results ascertained during prospective cohort studies and routine clinical care. CCR5-Δ32 genotype was determined by polymerase chain reaction, followed by single-stranded conformational polymorphism analysis. Results: Hypertension and conditions attributable to hemophilia were the only diagnoses frequently found in clinical records of CCR5-Δ32/Δ32 study subjects. Based on blood pressure measurement and treatment history, CCR5-Δ32/Δ32 homozygotes had a 2.8-fold higher prevalence of hypertension than age-matched CCR5-+/+ study subjects (95% confidence interval [CI], 1.2-6.4; p = .01); none of the homozygotes had severe hypertension. Hematologic measures were generally similar across the genotypes, but total lymphocyte counts were ~20% higher in CCR5-Δ32/Δ32 study subjects than in CCR5-+/+ study subjects (p < .05). Among patients with hemophilia who were infected with hepatitis C virus (HCV), mean alanine aminotransferase levels were 117% higher among CCR5-Δ32/Δ32 homozygotes (p < .05), but serum HCV levels did not differ by CCR5-Δ32 genotype. CCR5- Δ32/Δ32 homozygous study subjects had a lower prevalence of antibodies to measles virus than those with other genotypes, but this association was not confirmed in a group of blood donors. The prevalence of antibodies to nine other common viruses, HBV, and HCV was not related to CCR5 genotype. Conclusions: CCR5-Δ32/Δ32 homozygotes are generally similar to wild-type persons. Confirmatory investigations are required to determine whether hypertension, increased lymphocyte counts, and higher hepatic enzyme levels in the presence of HCV infection represent true phenotypic expressions of this genotype. CCR5-Δ32/Δ32 homozygosity does not provide broad protection against viral infections.

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DO - 10.1097/00042560-199909010-00010

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JO - Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology

JF - Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology

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ER -

Phenotypic expressions of CCR5-Δ32/Δ32 homozygosity

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