TY - JOUR
T1 - Phospholipid FA of piezophilic bacteria from the deep sea
AU - Fang, Jiasong
AU - Chan, Olivia
AU - Kato, Chiaki
AU - Sato, Takako
AU - Peeples, Tonya
AU - Niggemeyer, Karl
N1 - Funding Information:
We thank Lacey Bilden for laboratory assistance. We thank Dr. Howard Knapp, Dr. Colin Ratledge, and two anonymous reviewers whose constructive comments improved the manuscript. This work was supported by the National Science Foundation and National Aeronautics and Space Administration.
PY - 2003/8/1
Y1 - 2003/8/1
N2 - Phospholipid FA (PLFA) profiles were determined on four piezophilic bacteria from the deep sea: Moritella japonica DSK1, Shewanella violacea DSS12, S. benthica DB6705, and S. benthica DB21MT-2. The total concentrations of PLFA were higher in strains grown at low pressure (DSK1, 10 MPa, 27.0 mg/g dry wt cells; DSS12, 50 MPa, 24.0 mg/g), and lower in strains grown at high pressure (DB6705, 85 MPa, 1.9 mg/g; DB21MT-2, 100 MPa, 3.0 mg/g). The piezophilic bacteria were characterized by a high abundance of unsaturated FA (62-73% of total FA). In particular, PUFA were detected in all piezophiles examined. Moritella japonica DSK1 produced 22:6n-3 (DHA), whereas the three Shewanella strains produced 20:5n-3 (EPA) with trace amounts of DHA. The detection of low levels of the medium-chain-length PUFA 18:2n-6 and 18:3 (DSK1) and 20:2 (DB6705 and DB21 MT-2) suggests that the biosynthesis of EPA and DHA may be regulated by the formation and desaturation of di- and tri-unsaturated FA.
AB - Phospholipid FA (PLFA) profiles were determined on four piezophilic bacteria from the deep sea: Moritella japonica DSK1, Shewanella violacea DSS12, S. benthica DB6705, and S. benthica DB21MT-2. The total concentrations of PLFA were higher in strains grown at low pressure (DSK1, 10 MPa, 27.0 mg/g dry wt cells; DSS12, 50 MPa, 24.0 mg/g), and lower in strains grown at high pressure (DB6705, 85 MPa, 1.9 mg/g; DB21MT-2, 100 MPa, 3.0 mg/g). The piezophilic bacteria were characterized by a high abundance of unsaturated FA (62-73% of total FA). In particular, PUFA were detected in all piezophiles examined. Moritella japonica DSK1 produced 22:6n-3 (DHA), whereas the three Shewanella strains produced 20:5n-3 (EPA) with trace amounts of DHA. The detection of low levels of the medium-chain-length PUFA 18:2n-6 and 18:3 (DSK1) and 20:2 (DB6705 and DB21 MT-2) suggests that the biosynthesis of EPA and DHA may be regulated by the formation and desaturation of di- and tri-unsaturated FA.
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U2 - 10.1007/s11745-003-1140-7
DO - 10.1007/s11745-003-1140-7
M3 - Article
C2 - 14577669
AN - SCOPUS:0141481802
SN - 0024-4201
VL - 38
SP - 885
EP - 887
JO - Lipids
JF - Lipids
IS - 8
ER -