Photoaffinity labelling of a nuclear matrix nucleoside triphosphatase and its modulation in the acute-phase response

Gary A. Clawson; Jane Button; Edward A. Smuckler

doi:10.1016/S0014-4827(85)80046-X

Photoaffinity labelling of a nuclear matrix nucleoside triphosphatase and its modulation in the acute-phase response

Gary A. Clawson, Jane Button, Edward A. Smuckler

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Photoaffinity labelling has been used to identify the major nuclear matrix nucleoside triphosphatase (NTPase) as a 46 kD polypeptide, which appears to represent the same polypeptide photolabelled in nuclear envelope. Nuclear matrix NTPase and its photolabelling were selectively decreased in the acute phase response of rat liver, which also encompasses decreases (30%) in RNA transport in vitro and nuclear envelope NTPase. These results, and quantitative considerations suggest that the NTPase correlatively linked to RNA transport is not solubilized by detergents; it appears to represent a nuclear matrix component.

Original language	English (US)
Pages (from-to)	171-175
Number of pages	5
Journal	Experimental Cell Research
Volume	159
Issue number	1
DOIs	https://doi.org/10.1016/S0014-4827(85)80046-X
State	Published - Jul 1985

All Science Journal Classification (ASJC) codes

Cell Biology

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10.1016/S0014-4827(85)80046-X

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title = "Photoaffinity labelling of a nuclear matrix nucleoside triphosphatase and its modulation in the acute-phase response",

abstract = "Photoaffinity labelling has been used to identify the major nuclear matrix nucleoside triphosphatase (NTPase) as a 46 kD polypeptide, which appears to represent the same polypeptide photolabelled in nuclear envelope. Nuclear matrix NTPase and its photolabelling were selectively decreased in the acute phase response of rat liver, which also encompasses decreases (30%) in RNA transport in vitro and nuclear envelope NTPase. These results, and quantitative considerations suggest that the NTPase correlatively linked to RNA transport is not solubilized by detergents; it appears to represent a nuclear matrix component.",

author = "Clawson, {Gary A.} and Jane Button and Smuckler, {Edward A.}",

note = "Funding Information: The authors wish to thank David A. Geller for editorial assistance. This work was supported in part by USPHS grants CA21141 and AM19843.",

year = "1985",

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doi = "10.1016/S0014-4827(85)80046-X",

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T1 - Photoaffinity labelling of a nuclear matrix nucleoside triphosphatase and its modulation in the acute-phase response

AU - Clawson, Gary A.

AU - Button, Jane

AU - Smuckler, Edward A.

N1 - Funding Information: The authors wish to thank David A. Geller for editorial assistance. This work was supported in part by USPHS grants CA21141 and AM19843.

PY - 1985/7

Y1 - 1985/7

N2 - Photoaffinity labelling has been used to identify the major nuclear matrix nucleoside triphosphatase (NTPase) as a 46 kD polypeptide, which appears to represent the same polypeptide photolabelled in nuclear envelope. Nuclear matrix NTPase and its photolabelling were selectively decreased in the acute phase response of rat liver, which also encompasses decreases (30%) in RNA transport in vitro and nuclear envelope NTPase. These results, and quantitative considerations suggest that the NTPase correlatively linked to RNA transport is not solubilized by detergents; it appears to represent a nuclear matrix component.

AB - Photoaffinity labelling has been used to identify the major nuclear matrix nucleoside triphosphatase (NTPase) as a 46 kD polypeptide, which appears to represent the same polypeptide photolabelled in nuclear envelope. Nuclear matrix NTPase and its photolabelling were selectively decreased in the acute phase response of rat liver, which also encompasses decreases (30%) in RNA transport in vitro and nuclear envelope NTPase. These results, and quantitative considerations suggest that the NTPase correlatively linked to RNA transport is not solubilized by detergents; it appears to represent a nuclear matrix component.

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Photoaffinity labelling of a nuclear matrix nucleoside triphosphatase and its modulation in the acute-phase response

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