Photoautotrophic hydrogen production by eukaryotic microalgae under aerobic conditions

Jae Hoon Hwang; Hyun Chul Kim; Jeong A. Choi; R. A.I. Abou-Shanab; Brian A. Dempsey; John M. Regan; Jung Rae Kim; Hocheol Song; In Hyun Nam; Su Nam Kim; Woojung Lee; Donghee Park; Yongje Kim; Jaeyoung Choi; Min Kyu Ji; Woosik Jung; Byong Hun Jeon

doi:10.1038/ncomms4234

Photoautotrophic hydrogen production by eukaryotic microalgae under aerobic conditions

Jae Hoon Hwang, Hyun Chul Kim, Jeong A. Choi, R. A.I. Abou-Shanab, Brian A. Dempsey, John M. Regan, Jung Rae Kim, Hocheol Song, In Hyun Nam, Su Nam Kim, Woojung Lee, Donghee Park, Yongje Kim, Jaeyoung Choi, Min Kyu Ji, Woosik Jung, Byong Hun Jeon

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104 Scopus citations

Abstract

Eukaryotic algae and cyanobacteria produce hydrogen under anaerobic and limited aerobic conditions. Here we show that novel microalgal strains (Chlorella vulgaris YSL01 and YSL16) upregulate the expression of the hydrogenase gene (HYDA) and simultaneously produce hydrogen through photosynthesis, using CO 2 as the sole source of carbon under aerobic conditions with continuous illumination. We employ dissolved oxygen regimes that represent natural aquatic conditions for microalgae. The experimental expression of HYDA and the specific activity of hydrogenase demonstrate that C. vulgaris YSL01 and YSL16 enzymatically produce hydrogen, even under atmospheric conditions, which was previously considered infeasible. Photoautotrophic H 2 production has important implications for assessing ecological and algae-based photolysis.

Original language	English (US)
Article number	3234
Journal	Nature communications
Volume	5
DOIs	https://doi.org/10.1038/ncomms4234
State	Published - Feb 4 2014

All Science Journal Classification (ASJC) codes

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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Hwang, J. H., Kim, H. C., Choi, J. A., Abou-Shanab, R. A. I., Dempsey, B. A., Regan, J. M., Kim, J. R., Song, H., Nam, I. H., Kim, S. N., Lee, W., Park, D., Kim, Y., Choi, J., Ji, M. K., Jung, W., & Jeon, B. H. (2014). Photoautotrophic hydrogen production by eukaryotic microalgae under aerobic conditions. Nature communications, 5, Article 3234. https://doi.org/10.1038/ncomms4234

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title = "Photoautotrophic hydrogen production by eukaryotic microalgae under aerobic conditions",

abstract = "Eukaryotic algae and cyanobacteria produce hydrogen under anaerobic and limited aerobic conditions. Here we show that novel microalgal strains (Chlorella vulgaris YSL01 and YSL16) upregulate the expression of the hydrogenase gene (HYDA) and simultaneously produce hydrogen through photosynthesis, using CO 2 as the sole source of carbon under aerobic conditions with continuous illumination. We employ dissolved oxygen regimes that represent natural aquatic conditions for microalgae. The experimental expression of HYDA and the specific activity of hydrogenase demonstrate that C. vulgaris YSL01 and YSL16 enzymatically produce hydrogen, even under atmospheric conditions, which was previously considered infeasible. Photoautotrophic H 2 production has important implications for assessing ecological and algae-based photolysis.",

author = "Hwang, {Jae Hoon} and Kim, {Hyun Chul} and Choi, {Jeong A.} and Abou-Shanab, {R. A.I.} and Dempsey, {Brian A.} and Regan, {John M.} and Kim, {Jung Rae} and Hocheol Song and Nam, {In Hyun} and Kim, {Su Nam} and Woojung Lee and Donghee Park and Yongje Kim and Jaeyoung Choi and Ji, {Min Kyu} and Woosik Jung and Jeon, {Byong Hun}",

note = "Funding Information: This work was supported by the Senior Researchers program (National Research Foundation of Korea, 2013069183), the Eco-Innovation research project (Global-Top project, 2012001090001) of the Korea Ministry of Environment, and the Korea Institute of Science & Technology (No. 2Z03860).",

year = "2014",

month = feb,

day = "4",

doi = "10.1038/ncomms4234",

language = "English (US)",

volume = "5",

journal = "Nature communications",

issn = "2041-1723",

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T1 - Photoautotrophic hydrogen production by eukaryotic microalgae under aerobic conditions

AU - Hwang, Jae Hoon

AU - Kim, Hyun Chul

AU - Choi, Jeong A.

AU - Abou-Shanab, R. A.I.

AU - Dempsey, Brian A.

AU - Regan, John M.

AU - Kim, Jung Rae

AU - Song, Hocheol

AU - Nam, In Hyun

AU - Kim, Su Nam

AU - Lee, Woojung

AU - Park, Donghee

AU - Kim, Yongje

AU - Choi, Jaeyoung

AU - Ji, Min Kyu

AU - Jung, Woosik

AU - Jeon, Byong Hun

N1 - Funding Information: This work was supported by the Senior Researchers program (National Research Foundation of Korea, 2013069183), the Eco-Innovation research project (Global-Top project, 2012001090001) of the Korea Ministry of Environment, and the Korea Institute of Science & Technology (No. 2Z03860).

PY - 2014/2/4

Y1 - 2014/2/4

N2 - Eukaryotic algae and cyanobacteria produce hydrogen under anaerobic and limited aerobic conditions. Here we show that novel microalgal strains (Chlorella vulgaris YSL01 and YSL16) upregulate the expression of the hydrogenase gene (HYDA) and simultaneously produce hydrogen through photosynthesis, using CO 2 as the sole source of carbon under aerobic conditions with continuous illumination. We employ dissolved oxygen regimes that represent natural aquatic conditions for microalgae. The experimental expression of HYDA and the specific activity of hydrogenase demonstrate that C. vulgaris YSL01 and YSL16 enzymatically produce hydrogen, even under atmospheric conditions, which was previously considered infeasible. Photoautotrophic H 2 production has important implications for assessing ecological and algae-based photolysis.

AB - Eukaryotic algae and cyanobacteria produce hydrogen under anaerobic and limited aerobic conditions. Here we show that novel microalgal strains (Chlorella vulgaris YSL01 and YSL16) upregulate the expression of the hydrogenase gene (HYDA) and simultaneously produce hydrogen through photosynthesis, using CO 2 as the sole source of carbon under aerobic conditions with continuous illumination. We employ dissolved oxygen regimes that represent natural aquatic conditions for microalgae. The experimental expression of HYDA and the specific activity of hydrogenase demonstrate that C. vulgaris YSL01 and YSL16 enzymatically produce hydrogen, even under atmospheric conditions, which was previously considered infeasible. Photoautotrophic H 2 production has important implications for assessing ecological and algae-based photolysis.

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Photoautotrophic hydrogen production by eukaryotic microalgae under aerobic conditions

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