Phylogenetic relationships of Staphylococcus aureus from bovine mastitis based on coagulase gene polymorphism

C. Su; I. Kanevsky; B. M. Jayarao; L. M. Sordillo

doi:10.1016/S0378-1135(99)00139-X

Phylogenetic relationships of Staphylococcus aureus from bovine mastitis based on coagulase gene polymorphism

C. Su, I. Kanevsky, B. M. Jayarao, L. M. Sordillo

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Coagulase gene restriction fragment length polymorphism (RFLP) patterns were analyzed to determine the phylogenetic relationship among isolates of Staphylococcus aureus from the Czech Republic (n=27), France (n=48), Korea (n=115) and the United States (n=278). A total of 468 isolates of S. aureus were subtyped into 41 coagulase genotypes. Cluster analysis placed the 41 types into nine clusters. Eighteen API Staph profiles were determined for 102 S. aureus isolates representing 1 to 4 isolates of each coagulase type. The results of the study suggest that based on coagulase gene RFLP analysis, several genetic variants of S. aureus are prevalent. Comparison of coagulase and API Staph profiles indicated that the two identification system were independent of each other.

Original language	English (US)
Pages (from-to)	53-58
Number of pages	6
Journal	Veterinary Microbiology
Volume	71
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-1135(99)00139-X
State	Published - Jan 2000

All Science Journal Classification (ASJC) codes

Microbiology
General Veterinary

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10.1016/S0378-1135(99)00139-X

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@article{be3869cc22c84b129558666091373bcc,

title = "Phylogenetic relationships of Staphylococcus aureus from bovine mastitis based on coagulase gene polymorphism",

abstract = "Coagulase gene restriction fragment length polymorphism (RFLP) patterns were analyzed to determine the phylogenetic relationship among isolates of Staphylococcus aureus from the Czech Republic (n=27), France (n=48), Korea (n=115) and the United States (n=278). A total of 468 isolates of S. aureus were subtyped into 41 coagulase genotypes. Cluster analysis placed the 41 types into nine clusters. Eighteen API Staph profiles were determined for 102 S. aureus isolates representing 1 to 4 isolates of each coagulase type. The results of the study suggest that based on coagulase gene RFLP analysis, several genetic variants of S. aureus are prevalent. Comparison of coagulase and API Staph profiles indicated that the two identification system were independent of each other.",

author = "C. Su and I. Kanevsky and Jayarao, {B. M.} and Sordillo, {L. M.}",

note = "Funding Information: This research was supported in part by agricultural research funds administered by the Pennsylvania Department of Agriculture (ME445126) and the USDA-BARD Grants Program (US-2648-95). We appreciate Drs. B. Poutrel, J.W. Yoon and O. Skardova for providing S. aureus isolates from France, Korea and the Czech Republic, respectively. ",

year = "2000",

month = jan,

doi = "10.1016/S0378-1135(99)00139-X",

language = "English (US)",

volume = "71",

pages = "53--58",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier B.V.",

number = "1-2",

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TY - JOUR

T1 - Phylogenetic relationships of Staphylococcus aureus from bovine mastitis based on coagulase gene polymorphism

AU - Su, C.

AU - Kanevsky, I.

AU - Jayarao, B. M.

AU - Sordillo, L. M.

N1 - Funding Information: This research was supported in part by agricultural research funds administered by the Pennsylvania Department of Agriculture (ME445126) and the USDA-BARD Grants Program (US-2648-95). We appreciate Drs. B. Poutrel, J.W. Yoon and O. Skardova for providing S. aureus isolates from France, Korea and the Czech Republic, respectively.

PY - 2000/1

Y1 - 2000/1

N2 - Coagulase gene restriction fragment length polymorphism (RFLP) patterns were analyzed to determine the phylogenetic relationship among isolates of Staphylococcus aureus from the Czech Republic (n=27), France (n=48), Korea (n=115) and the United States (n=278). A total of 468 isolates of S. aureus were subtyped into 41 coagulase genotypes. Cluster analysis placed the 41 types into nine clusters. Eighteen API Staph profiles were determined for 102 S. aureus isolates representing 1 to 4 isolates of each coagulase type. The results of the study suggest that based on coagulase gene RFLP analysis, several genetic variants of S. aureus are prevalent. Comparison of coagulase and API Staph profiles indicated that the two identification system were independent of each other.

AB - Coagulase gene restriction fragment length polymorphism (RFLP) patterns were analyzed to determine the phylogenetic relationship among isolates of Staphylococcus aureus from the Czech Republic (n=27), France (n=48), Korea (n=115) and the United States (n=278). A total of 468 isolates of S. aureus were subtyped into 41 coagulase genotypes. Cluster analysis placed the 41 types into nine clusters. Eighteen API Staph profiles were determined for 102 S. aureus isolates representing 1 to 4 isolates of each coagulase type. The results of the study suggest that based on coagulase gene RFLP analysis, several genetic variants of S. aureus are prevalent. Comparison of coagulase and API Staph profiles indicated that the two identification system were independent of each other.

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U2 - 10.1016/S0378-1135(99)00139-X

DO - 10.1016/S0378-1135(99)00139-X

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EP - 58

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 1-2

ER -

Phylogenetic relationships of Staphylococcus aureus from bovine mastitis based on coagulase gene polymorphism

Abstract

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