TY - JOUR
T1 - Phylogeographic analyses suggest multiple lineages of Crystallaria asprella (Percidae: Etheostominae)
AU - Morrison, Cheryl L.
AU - Lemarié, David P.
AU - Wood, Robert M.
AU - King, Tim L.
N1 - Funding Information:
The authors are greatly indebted to Mary Burnham-Curtis, Cathy Dayton, Ellen Grey, and Brian Sloss for specimen donation; to Shannon Julian, Robin Johnson, Mike Eackles and Ellen Grey for laboratory assistance, and to John Young for creating the map of our study sites. Funding was provided by the US Geological Survey®s Lee-town Science Center – Aquatic Ecology Branch, and the Quick Response Program administered by the USGS-BRD Central Region. Region 5, US Fish and Wildlife Service contributed funding to broaden the scope of the study. The quality of this manuscript was greatly improved through the thoughtful suggestions kindly provided by Dr. Alfried Vogler and two anonymous reviewers.
PY - 2006/2
Y1 - 2006/2
N2 - The crystal darter, Crystallaria asprella, exists in geographically isolated populations that may be glacial relicts from its former, wide distribution in the Eastern U.S. An initial phylogeographic survey of C. asprella based upon the mitochondrial cytochrome b (cyt b) gene indicated that there were at least four distinct populations within the species: Ohio River basin, Upper Mississippi River, Gulf coast, and lower Mississippi River. In particular, the most divergent population was the most recently discovered, from the Elk River, WV, in the Ohio River basin, and it was postulated that this population represents an undescribed, potentially threatened species. However, differentiation observed at a single gene region is generally not considered sufficient evidence to establish taxonomic status. In the present study, nucleotide variation at the mitochondrial control region and a nuclear S7 ribosomal gene intron were compared to provide independent verification of phylogeographic results between individuals collected from the same five disjunct populations previously surveyed. Variation between populations at the control region was substantial (except between Gulf drainages) and was concordant with patterns of sequence divergence from cyt b. Only the Elk River population was resolved as monophyletic based upon nuclear S7, but significant differences based upon ΦST statistics were observed between most populations. Morphometric data were consistent with molecular data regarding the distinctiveness of the Elk River population. It is proposed that populations of C. asprella consist of at least four distinct population segments, and that the Elk River group likely constitutes a distinct species.
AB - The crystal darter, Crystallaria asprella, exists in geographically isolated populations that may be glacial relicts from its former, wide distribution in the Eastern U.S. An initial phylogeographic survey of C. asprella based upon the mitochondrial cytochrome b (cyt b) gene indicated that there were at least four distinct populations within the species: Ohio River basin, Upper Mississippi River, Gulf coast, and lower Mississippi River. In particular, the most divergent population was the most recently discovered, from the Elk River, WV, in the Ohio River basin, and it was postulated that this population represents an undescribed, potentially threatened species. However, differentiation observed at a single gene region is generally not considered sufficient evidence to establish taxonomic status. In the present study, nucleotide variation at the mitochondrial control region and a nuclear S7 ribosomal gene intron were compared to provide independent verification of phylogeographic results between individuals collected from the same five disjunct populations previously surveyed. Variation between populations at the control region was substantial (except between Gulf drainages) and was concordant with patterns of sequence divergence from cyt b. Only the Elk River population was resolved as monophyletic based upon nuclear S7, but significant differences based upon ΦST statistics were observed between most populations. Morphometric data were consistent with molecular data regarding the distinctiveness of the Elk River population. It is proposed that populations of C. asprella consist of at least four distinct population segments, and that the Elk River group likely constitutes a distinct species.
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U2 - 10.1007/s10592-005-5681-8
DO - 10.1007/s10592-005-5681-8
M3 - Article
AN - SCOPUS:33644507988
SN - 1566-0621
VL - 7
SP - 129
EP - 147
JO - Conservation Genetics
JF - Conservation Genetics
IS - 1
ER -