Pigment epithelium derived factor (PEDF), A candidate for autocrine regulation of retinal pigment epithelial cells

J. Feher; F. Malchiodi Albedi; G. Formisano; R. Perilli; G. Scorcia; S. Pintucci; S. Caiazra; Joyce Tombran-Tink

Pigment epithelium derived factor (PEDF), A candidate for autocrine regulation of retinal pigment epithelial cells

J. Feher, F. Malchiodi Albedi, G. Formisano, R. Perilli, G. Scorcia, S. Pintucci, S. Caiazra, Joyce Tombran-Tink

Research output: Contribution to journal › Article › peer-review

Abstract

Pyrpose. The effects of PEDF on rat retinal cells were studied, Methods. Primary cultures of lewborn albino rat retinas were treated with PEDF (500 ng/ml) at 2 DIV. At 9 DIV, cultures were studied by phase contrast nicroscopy, SEM, TEM and immune-fluorescence techniques. Results. PEDF-treated cultures showed more numerous and larger pigment epithelial cells, with several cell-cell contacts, and a higher amount of more mature pigment granules than in controls. Conclusions. PEDF promotes survival and differentiation of pigment epithelial cells. These findings show that PEDF may be one of the factors of the autocrine-paracrine system whch operates in the pigment epithelium-photoreceptor complex.

Original language	English (US)
Journal	Investigative Ophthalmology and Visual Science
Volume	38
Issue number	4
State	Published - Dec 1 1997

All Science Journal Classification (ASJC) codes

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

Cite this

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title = "Pigment epithelium derived factor (PEDF), A candidate for autocrine regulation of retinal pigment epithelial cells",

abstract = "Pyrpose. The effects of PEDF on rat retinal cells were studied, Methods. Primary cultures of lewborn albino rat retinas were treated with PEDF (500 ng/ml) at 2 DIV. At 9 DIV, cultures were studied by phase contrast nicroscopy, SEM, TEM and immune-fluorescence techniques. Results. PEDF-treated cultures showed more numerous and larger pigment epithelial cells, with several cell-cell contacts, and a higher amount of more mature pigment granules than in controls. Conclusions. PEDF promotes survival and differentiation of pigment epithelial cells. These findings show that PEDF may be one of the factors of the autocrine-paracrine system whch operates in the pigment epithelium-photoreceptor complex.",

author = "J. Feher and Albedi, {F. Malchiodi} and G. Formisano and R. Perilli and G. Scorcia and S. Pintucci and S. Caiazra and Joyce Tombran-Tink",

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language = "English (US)",

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journal = "Investigative Ophthalmology and Visual Science",

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TY - JOUR

T1 - Pigment epithelium derived factor (PEDF), A candidate for autocrine regulation of retinal pigment epithelial cells

AU - Feher, J.

AU - Albedi, F. Malchiodi

AU - Formisano, G.

AU - Perilli, R.

AU - Scorcia, G.

AU - Pintucci, S.

AU - Caiazra, S.

AU - Tombran-Tink, Joyce

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Pyrpose. The effects of PEDF on rat retinal cells were studied, Methods. Primary cultures of lewborn albino rat retinas were treated with PEDF (500 ng/ml) at 2 DIV. At 9 DIV, cultures were studied by phase contrast nicroscopy, SEM, TEM and immune-fluorescence techniques. Results. PEDF-treated cultures showed more numerous and larger pigment epithelial cells, with several cell-cell contacts, and a higher amount of more mature pigment granules than in controls. Conclusions. PEDF promotes survival and differentiation of pigment epithelial cells. These findings show that PEDF may be one of the factors of the autocrine-paracrine system whch operates in the pigment epithelium-photoreceptor complex.

AB - Pyrpose. The effects of PEDF on rat retinal cells were studied, Methods. Primary cultures of lewborn albino rat retinas were treated with PEDF (500 ng/ml) at 2 DIV. At 9 DIV, cultures were studied by phase contrast nicroscopy, SEM, TEM and immune-fluorescence techniques. Results. PEDF-treated cultures showed more numerous and larger pigment epithelial cells, with several cell-cell contacts, and a higher amount of more mature pigment granules than in controls. Conclusions. PEDF promotes survival and differentiation of pigment epithelial cells. These findings show that PEDF may be one of the factors of the autocrine-paracrine system whch operates in the pigment epithelium-photoreceptor complex.

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Pigment epithelium derived factor (PEDF), A candidate for autocrine regulation of retinal pigment epithelial cells

Abstract

All Science Journal Classification (ASJC) codes

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