TY - JOUR
T1 - Plant single-cell and single-cell-type metabolomics
AU - Misra, Biswapriya B.
AU - Assmann, Sarah M.
AU - Chen, Sixue
N1 - Funding Information:
This work was supported by the US National Science Foundation grants NSF-MCB-0817954, NSF-MCB-1157921, and NSF-IOS 1025837 to S.M.A. and NSF grants NSF-MCB-0818051, NSF-MCB-1158000, and NSF-CAREER-0845162 to S.C. We thank Mengmeng Zhu for helpful comments on the manuscript. We apologize to all the colleagues whose work could not be cited owing to space constraints. The authors appreciate the comments of three anonymous reviewers that improved this article.
Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - In conjunction with genomics, transcriptomics, and proteomics, plant metabolomics is providing large data sets that are paving the way towards a comprehensive and holistic understanding of plant growth, development, defense, and productivity. However, dilution effects from organ- and tissue-based sampling of metabolomes have limited our understanding of the intricate regulation of metabolic pathways and networks at the cellular level. Recent advances in metabolomics methodologies, along with the post-genomic expansion of bioinformatics knowledge and functional genomics tools, have allowed the gathering of enriched information on individual cells and single cell types. Here we review progress, current status, opportunities, and challenges presented by single cell-based metabolomics research in plants.
AB - In conjunction with genomics, transcriptomics, and proteomics, plant metabolomics is providing large data sets that are paving the way towards a comprehensive and holistic understanding of plant growth, development, defense, and productivity. However, dilution effects from organ- and tissue-based sampling of metabolomes have limited our understanding of the intricate regulation of metabolic pathways and networks at the cellular level. Recent advances in metabolomics methodologies, along with the post-genomic expansion of bioinformatics knowledge and functional genomics tools, have allowed the gathering of enriched information on individual cells and single cell types. Here we review progress, current status, opportunities, and challenges presented by single cell-based metabolomics research in plants.
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U2 - 10.1016/j.tplants.2014.05.005
DO - 10.1016/j.tplants.2014.05.005
M3 - Review article
C2 - 24946988
AN - SCOPUS:84908204987
SN - 1360-1385
VL - 19
SP - 637
EP - 646
JO - Trends in Plant Science
JF - Trends in Plant Science
IS - 10
ER -