TY - JOUR
T1 - Plasma volume variation across the menstrual cycle among healthy women of reproductive age
T2 - A prospective cohort study
AU - Aguree, Sixtus
AU - Bethancourt, Hilary J.
AU - Taylor, Leigh A.
AU - Rosinger, Asher Y.
AU - Gernand, Alison D.
N1 - Funding Information:
We thank the medical staff, especially Cyndi Flanagan, at the Clinical Research Center of the Pennsylvania State University's Clinical and Translational Science Institute. We thank Dr. James A. Pawelczyk, Associate Professor of Physiology and Kinesiology at The Pennsylvania State University, for his expert technical assistance on the use of indocyanine green. We also thank Dr. Mary Jane De Souza and the members of the Women's Health and Exercise Lab that measured reproductive hormones. The project described was supported by the National Center for Advancing Translational Sciences, National Institutes of Health, through Grant UL1 TR002014. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.
Funding Information:
College of Health and Human Development, The Pennsylvania State University. We thank the medical staff, especially Cyndi Flanagan, at the Clinical Research Center of the Pennsylvania State University's Clinical and Translational Science Institute. We thank Dr. James A. Pawelczyk, Associate Professor of Physiology and Kinesiology at The Pennsylvania State University, for his expert technical assistance on the use of indocyanine green.?We also thank Dr. Mary Jane De Souza and the members of the Women's Health and Exercise Lab that measured reproductive hormones. The project described was supported by the National Center for Advancing Translational Sciences, National Institutes of Health, through Grant UL1 TR002014. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.
Publisher Copyright:
© 2020 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Increases in reproductive hormones like estrogen, play an important role in the remarkable increases in plasma volume observed in pregnancy. Accurate estimates of plasma volume expansion during pregnancy depend on correctly timing and measuring plasma volume in nonpregnant women. However, to date, there is no consensus on the pattern of plasma volume across the menstrual cycle. We prospectively measured plasma volume in 45 women across a single menstrual cycle. A urine-based fertility monitor was used to time three clinic visits to distinct points in the menstrual cycle: the early follicular phase (~day 2), periovulation (~day 12), and the mid-point of the luteal phase (~day 21)—based on a 28-day cycle length. Healthy women aged 18–41 years with regular menstrual cycles and a healthy body weight were enrolled in the study. At each visit, blood samples were collected before and after injection of 0.25 mg/kg body weight of indocyanine green dye (ICG). Pre- and post-ICG injection plasma samples were used to measure plasma volume. Preinjection samples were used to measure ovarian hormones and plasma osmolality. Mean plasma volume was highest during the early follicular phase (2,276 ± 478 ml); it declined to 2,232 ± 509 ml by the late follicular phase and to 2,228 ± 502 ml by the midluteal phase. This study found that overall variations in plasma volume are small across the menstrual cycle. Therefore, in clinical practice and research, the menstrual cycle phase may not be an important consideration when evaluating plasma volume among women of reproductive age.
AB - Increases in reproductive hormones like estrogen, play an important role in the remarkable increases in plasma volume observed in pregnancy. Accurate estimates of plasma volume expansion during pregnancy depend on correctly timing and measuring plasma volume in nonpregnant women. However, to date, there is no consensus on the pattern of plasma volume across the menstrual cycle. We prospectively measured plasma volume in 45 women across a single menstrual cycle. A urine-based fertility monitor was used to time three clinic visits to distinct points in the menstrual cycle: the early follicular phase (~day 2), periovulation (~day 12), and the mid-point of the luteal phase (~day 21)—based on a 28-day cycle length. Healthy women aged 18–41 years with regular menstrual cycles and a healthy body weight were enrolled in the study. At each visit, blood samples were collected before and after injection of 0.25 mg/kg body weight of indocyanine green dye (ICG). Pre- and post-ICG injection plasma samples were used to measure plasma volume. Preinjection samples were used to measure ovarian hormones and plasma osmolality. Mean plasma volume was highest during the early follicular phase (2,276 ± 478 ml); it declined to 2,232 ± 509 ml by the late follicular phase and to 2,228 ± 502 ml by the midluteal phase. This study found that overall variations in plasma volume are small across the menstrual cycle. Therefore, in clinical practice and research, the menstrual cycle phase may not be an important consideration when evaluating plasma volume among women of reproductive age.
UR - http://www.scopus.com/inward/record.url?scp=85083949782&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85083949782&partnerID=8YFLogxK
U2 - 10.14814/phy2.14418
DO - 10.14814/phy2.14418
M3 - Article
C2 - 32323928
AN - SCOPUS:85083949782
SN - 2051-817X
VL - 8
JO - Physiological reports
JF - Physiological reports
IS - 8
M1 - e14418
ER -