Poly-vinyl pyrrolidone (PVP)-coated silver (Ag) nanoparticles (Ag-PVP): A potential to regulate chlamydia trachomatis inflammation

Abebayehu N. Yilma, Shree R. Singh, Saurabh Dixit, Gebremeskel G. Hidat, Vida A. Dennis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Chlamydia trachomatis is an important cause of sexually transmitted infection worldwide. Excessive inflammatory responses play a key role in its disease progression. Early intervention strategies that can reduce inflammatory responses could benefit control efforts in reducing the disease severity. Previously we reported that Ag-PVP reduced pro-inflammatory cytokines produced by C. trachomatis-infected mouse J774 macrophages. Here we reveal that Ag-PVP also exerts its anti-inflammatory effect on primary mouse bone marrow-derived macrophages (BMDM). The MTT assay showed that Ag-PVP was relatively non-toxic to BMDM (up to 6.25 μg/mL) over 72 hr. By ELISA we document that Ag-PVP (2.5 μg/mL) inhibited IL-6 (cytokine) and CXCL10 (chemokine) as produced by C. trachomatis-infected BMDM. Employing qRT-PCR we demonstrate that Ag-PVP down-regulated the expression of the surface receptors, TLR2 and NOD2, as triggered by C. trachomatis on J774 macrophages, suggesting that Ag-PVP may exert its anti-inflammatory effect by interfering with their expression. Our results show the anti-inflammatory effect of Ag-PVP on BMDM is not associated with cell death. Of importance our data demonstrates that Ag-PVP may mediate its antiinflammatory effect in infected macrophages by reducing macrophage surface receptors expression.

Original languageEnglish (US)
Title of host publicationTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Pages347-350
Number of pages4
StatePublished - 2013
EventNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 - Washington, DC, United States
Duration: May 12 2013May 16 2013

Publication series

NameTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Volume3

Other

OtherNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Country/TerritoryUnited States
CityWashington, DC
Period5/12/135/16/13

All Science Journal Classification (ASJC) codes

  • Biotechnology

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