Abstract
When non-proliferating 3T3-L1 fibroblasts were stimulated to differentiate into adipose cells, there was a dramatic increase in the intracellular level of the polyamine, spermidine. Addition of α-difluoromethylornithine, an inhibitor of polyamine biosynthesis, depleted the cellular polyamines and prevented triglyceride accumulation and differentiation. The inhibitory effect of α-difluoromethylorinithine was completely abolished by provision of spermidine or putrescine. This suggests that polyamines are needed in the processes of differentiation as well as their established requirement for cell growth.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 272-278 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 102 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 16 1981 |
All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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