Polycyclic aromatic hydrocarbon reference range levels in the U.S. population by measurement of urinary mono-hydroxy metabolites

James Grainger, Wenlin Huang, Zheng Li, Selvin Edwards, Charisse Walcott, Christopher Smith, Wayman Turner, Richard Wang, Donald G. Patterson

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We developed a gas chromatography/ isotope dilution high resolution mass spectrometry (GC/ID-HRMS) method for measuring 18 PAH metabolites representing 8 parent PAHs in 3 mL of urine at low part-per-trillion levels. We applied this method to the analysis of urine specimens from approximately 2400 people who participated in the National Health and Nutrition Examination Survey for the years 1999 and 2000 to determine levels for 14 PAH hydroxy metabolites of 7 parent compounds. Using this GC/ID-HRMS method, we found detectable concentrations for monohydroxy metabolite isomers fluorene, phenanthrene, fluoranthene, and pyrene, and for chrysene, benzo[c]phenanthrene, and benz[a]anthracene. Some mono-hydroxy metabolite isomers of chrysene, benzo[c]phenanthrene, and benz[a]anthracene exhibited low detection frequencies which did not allow for geometric mean calculations. From our study, we established a reference range for the targeted PAHs in the general U.S. population.

Original languageEnglish (US)
Pages (from-to)47-65
Number of pages19
JournalPolycyclic Aromatic Compounds
Volume25
Issue number1
DOIs
StatePublished - Jan 1 2005

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

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