@article{dc057da5c78a4eaaa50b1eaa90c2a917,
title = "Population structure and attribution of human clinical Campylobacter jejuni isolates from central Europe to livestock and environmental sources",
abstract = "Campylobacter jejuni is among the most prevalent causes of human bacterial gastroenteritis worldwide. Domesticated animals and, especially, chicken meat are considered to be the main sources of infections. However, the contribution of surface waters and wildlife in C. jejuni transmission to humans is not well understood. We have evaluated the source attribution potential of a six-gene multiplex PCR (mPCR) method coupled with STRUCTURE analysis on a set of 410 C. jejuni strains isolated from environment, livestock, food and humans in central Europe. Multiplex PCR fingerprints were analysed using Subclade prediction algorithm to classify them into six distinct mPCR clades. A subset of C. jejuni isolates (70%) was characterized by multilocus sequence typing (MLST) demonstrating 74% congruence between mPCR and MLST. The correspondence analysis of mPCR clades and sources of isolation indicated three distinct groups in the studied C. jejuni population—the first one associated with isolates from poultry, the second one with isolates from cattle, and the third one with isolates from the environment. The STRUCTURE analysis attributed 7.2% and 21.7% of human isolates to environmental sources based on MLST and mPCR fingerprints, respectively.",
author = "J. Kovac and B. Stessl and N. {\v C}ade{\v z} and I. Gruntar and M. Cimerman and K. Stingl and M. Lu{\v s}icky and M. Ocepek and M. Wagner and {Smole Mo{\v z}ina}, S.",
note = "Funding Information: The doctoral project of JK was partially funded by the European Union, the European Social Fund and the Ministry for Education, Science and Sport of the Republic of Slovenia in the framework of the operational programme for human resources development for the period 2007– 2013. Study was cofunded by the European Commission through the project PROMISE (FP7-265877) and the Slovenian Research Agency (CRP V4-1110, V4-1110 and J4-7608). The authors thank Dr. Sandra Jelovcan from the Austrian National Reference Laboratory for Campylobacter (Austrian Agency for Health and Food Safety; AGES), and Dr. Marija Trkov from the Centre for Clinical Microbiology (National Laboratory of Health, Environment and Food; NLZOH) for providing Campylobacter jejuni strains, as well as Diletta Di Marco for technical assistance. Funding Information: The doctoral project of JK was partially funded by the European Union, the European Social Fund and the Ministry for Education, Science and Sport of the Republic of Slovenia in the framework of the operational programme for human resources development for the period 2007–2013. Study was cofunded by the European Commission through the project PROMISE (FP7-265877) and the Slovenian Research Agency (CRP V4-1110, V4-1110 and J4-7608). The authors thank Dr. Sandra Jelovcan from the Austrian National Reference Laboratory for Campylobacter (Austrian Agency for Health and Food Safety; AGES), and Dr. Marija Trkov from the Centre for Clinical Microbiology (National Laboratory of Health, Environment and Food; NLZOH) for providing Campylobacter jejuni strains, as well as Diletta Di Marco for technical assistance. Publisher Copyright: {\textcopyright} 2017 Blackwell Verlag GmbH",
year = "2018",
month = feb,
doi = "10.1111/zph.12366",
language = "English (US)",
volume = "65",
pages = "51--58",
journal = "Zoonoses and Public Health",
issn = "1863-1959",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "1",
}
