Abstract
A growth hormone-dependent insulin-like growth factor (IGF) binding protein (IGFBP) purified from porcine serum specifically blocked the acute insulin-like effects of IGF-I on lipogenesis and glucose oxidation in porcine adipose tissue. This inhibition was dose dependent with half-maximal effective concentrations of IGFBP of 530 ng/ml for lipogenesis and 590 ng/ml for glucose oxidation in the presence of 10-8 M IGF-I. The IGFBP also caused decreased rates of lipogenesis following a 1-hr preincubation of tissue with IGF-I (10-8 M). The IGFBP had no effect on insulin action on porcine adipose tissue. These findings demonstrate the inhibitory effects of a highly purified porcine serum IGFBP on the biologic effects of IGF-I in vitro, and provide evidence that the growth hormone-dependent IGFBP blocks the acute insulin-like actions of IGF-I in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 315-319 |
| Number of pages | 5 |
| Journal | Proceedings of the Society for Experimental Biology and Medicine |
| Volume | 190 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1989 |
All Science Journal Classification (ASJC) codes
- General Biochemistry, Genetics and Molecular Biology