Post-translational regulation of FLC is mediated by an E3 ubiquitin ligase activity of SINAT5 in Arabidopsis

Bong Soo Park; Wan Gyu Sang; Song Yion Yeu; Yang Do Choi; Nam Chon Paek; Min Chul Kim; Jong Tae Song; Hak Soo Seo

doi:10.1016/j.plantsci.2007.06.001

Post-translational regulation of FLC is mediated by an E3 ubiquitin ligase activity of SINAT5 in Arabidopsis

Bong Soo Park, Wan Gyu Sang, Song Yion Yeu, Yang Do Choi, Nam Chon Paek, Min Chul Kim, Jong Tae Song, Hak Soo Seo

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Suppression of Flowering Locus C (FLC) is critical for the transition from the vegetative to reproductive phase in Arabidopsis. FLC represses the expression of several genes involved in floral induction and its transcription is positively or negatively regulated by FRIGIDA or by vernalization, respectively. However, the regulatory mechanisms for the level and stability of FLC protein throughout development are unknown. Here, we show that SINAT5 colocalizes with FLC in the nuclear bodies and that its zinc finger motif interacts directly with the MADS-box domain of FLC. In addition, it is shown that SINAT5 has an E3 ubiquitin ligase activity towards FLC as a substrate, suggesting that SINAT5 participates in the regulation of flowering time through the ubiquitin-mediated proteolysis of FLC.

Original language	English (US)
Pages (from-to)	269-275
Number of pages	7
Journal	Plant Science
Volume	173
Issue number	2
DOIs	https://doi.org/10.1016/j.plantsci.2007.06.001
State	Published - Aug 2007

All Science Journal Classification (ASJC) codes

Genetics
Agronomy and Crop Science
Plant Science

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10.1016/j.plantsci.2007.06.001

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@article{24103eca2a824975827fb4e81015c283,

title = "Post-translational regulation of FLC is mediated by an E3 ubiquitin ligase activity of SINAT5 in Arabidopsis",

abstract = "Suppression of Flowering Locus C (FLC) is critical for the transition from the vegetative to reproductive phase in Arabidopsis. FLC represses the expression of several genes involved in floral induction and its transcription is positively or negatively regulated by FRIGIDA or by vernalization, respectively. However, the regulatory mechanisms for the level and stability of FLC protein throughout development are unknown. Here, we show that SINAT5 colocalizes with FLC in the nuclear bodies and that its zinc finger motif interacts directly with the MADS-box domain of FLC. In addition, it is shown that SINAT5 has an E3 ubiquitin ligase activity towards FLC as a substrate, suggesting that SINAT5 participates in the regulation of flowering time through the ubiquitin-mediated proteolysis of FLC.",

author = "Park, {Bong Soo} and Sang, {Wan Gyu} and Yeu, {Song Yion} and Choi, {Yang Do} and Paek, {Nam Chon} and Kim, {Min Chul} and Song, {Jong Tae} and Seo, {Hak Soo}",

note = "Funding Information: This work was supported by Korea Research Foundation Grant funded by Korea Government (MOEHRD, Basic Research Promotion Fund) (KRF-2005-003-C00119) and by the Basic Research Program of the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. R01-2006-000-10035-0).",

year = "2007",

month = aug,

doi = "10.1016/j.plantsci.2007.06.001",

language = "English (US)",

volume = "173",

pages = "269--275",

journal = "Plant Science",

issn = "0168-9452",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Post-translational regulation of FLC is mediated by an E3 ubiquitin ligase activity of SINAT5 in Arabidopsis

AU - Park, Bong Soo

AU - Sang, Wan Gyu

AU - Yeu, Song Yion

AU - Choi, Yang Do

AU - Paek, Nam Chon

AU - Kim, Min Chul

AU - Song, Jong Tae

AU - Seo, Hak Soo

N1 - Funding Information: This work was supported by Korea Research Foundation Grant funded by Korea Government (MOEHRD, Basic Research Promotion Fund) (KRF-2005-003-C00119) and by the Basic Research Program of the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. R01-2006-000-10035-0).

PY - 2007/8

Y1 - 2007/8

N2 - Suppression of Flowering Locus C (FLC) is critical for the transition from the vegetative to reproductive phase in Arabidopsis. FLC represses the expression of several genes involved in floral induction and its transcription is positively or negatively regulated by FRIGIDA or by vernalization, respectively. However, the regulatory mechanisms for the level and stability of FLC protein throughout development are unknown. Here, we show that SINAT5 colocalizes with FLC in the nuclear bodies and that its zinc finger motif interacts directly with the MADS-box domain of FLC. In addition, it is shown that SINAT5 has an E3 ubiquitin ligase activity towards FLC as a substrate, suggesting that SINAT5 participates in the regulation of flowering time through the ubiquitin-mediated proteolysis of FLC.

AB - Suppression of Flowering Locus C (FLC) is critical for the transition from the vegetative to reproductive phase in Arabidopsis. FLC represses the expression of several genes involved in floral induction and its transcription is positively or negatively regulated by FRIGIDA or by vernalization, respectively. However, the regulatory mechanisms for the level and stability of FLC protein throughout development are unknown. Here, we show that SINAT5 colocalizes with FLC in the nuclear bodies and that its zinc finger motif interacts directly with the MADS-box domain of FLC. In addition, it is shown that SINAT5 has an E3 ubiquitin ligase activity towards FLC as a substrate, suggesting that SINAT5 participates in the regulation of flowering time through the ubiquitin-mediated proteolysis of FLC.

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DO - 10.1016/j.plantsci.2007.06.001

M3 - Article

AN - SCOPUS:34250901590

SN - 0168-9452

VL - 173

SP - 269

EP - 275

JO - Plant Science

JF - Plant Science

IS - 2

ER -

Post-translational regulation of FLC is mediated by an E3 ubiquitin ligase activity of SINAT5 in Arabidopsis

Abstract

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