TY - JOUR
T1 - Potential role of porcine reproductive and respiratory syndrome virus structural protein GP2 in apoptosis inhibition
AU - Pujhari, Sujit
AU - Baig, Tayyba T.
AU - Zakhartchouk, Alexander N.
PY - 2014
Y1 - 2014
N2 - Porcine reproductive and respiratory syndrome virus (PRRSV) is a serious threat to the pork industry, and its pathogenesis needs further investigations. To study the role of two structural proteins of PRRSV in virus-host cells interactions, two stable cell lines (MARC-2a and MARC-N) expressing GP2 and N proteins, respectively, were established. We induced apoptosis in these cells by treating them with staurosporine and found a significant reduction in the number of apoptotic cells in MARC-2a as compared to MARC-N and MARC-145 cells. In addition, we found significantly higher activities of transcriptional factors (NF-B and AP-1) in both cell lines as compared to MARC-145 (parent cells). Overall, our data suggest that, although both stable cell lines activate NF-B and AP-1, GP2 triggers the antiapoptotic process through an intermediate step that needs to be further investigated.
AB - Porcine reproductive and respiratory syndrome virus (PRRSV) is a serious threat to the pork industry, and its pathogenesis needs further investigations. To study the role of two structural proteins of PRRSV in virus-host cells interactions, two stable cell lines (MARC-2a and MARC-N) expressing GP2 and N proteins, respectively, were established. We induced apoptosis in these cells by treating them with staurosporine and found a significant reduction in the number of apoptotic cells in MARC-2a as compared to MARC-N and MARC-145 cells. In addition, we found significantly higher activities of transcriptional factors (NF-B and AP-1) in both cell lines as compared to MARC-145 (parent cells). Overall, our data suggest that, although both stable cell lines activate NF-B and AP-1, GP2 triggers the antiapoptotic process through an intermediate step that needs to be further investigated.
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U2 - 10.1155/2014/160505
DO - 10.1155/2014/160505
M3 - Article
C2 - 24511529
AN - SCOPUS:84893409443
SN - 2314-6133
VL - 2014
JO - BioMed Research International
JF - BioMed Research International
M1 - 160505
ER -