TY - JOUR
T1 - PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent Chromatin
AU - Nishioka, Kenichi
AU - Rice, Judd C.
AU - Sarma, Kavitha
AU - Erdjument-Bromage, Hediye
AU - Werner, Janis
AU - Wang, Yanming
AU - Chuikov, Sergei
AU - Valenzuela, Pablo
AU - Tempst, Paul
AU - Steward, Ruth
AU - Lis, John T.
AU - Allis, C. David
AU - Reinberg, Danny
N1 - Funding Information:
We thank members of the Allis, Lis, and Reinberg laboratories for helpful discussions and to Sherrie Hu for technical assistance. We thank Brian Schwartz for analyzing the distribution of RNA Polymerase II (regardless of CTD-phosphorylation) with respect to methyl H4-K20 on Drosophila polytene chromosomes (see cover). This work was supported by grants from NIH (GM-37120) and the Howard Hughes Medical Institute to D.R., NIH (GM-53512) to C.D.A., NIH (GM-25232) to J.T.L., NCI Cancer Center Grant P30 CA08748 to P.T., and an NIH training grant (GM-64279) to J.C.R. We also wish to thank Upstate Biotechnology, especially Mary Anne Jelineck and Rene Rice, for their support and development of the polyclonal methyl H4-K20 antibody. We thank Thomas Jenuwein for the use of the wild-type and Suv39h1/h2 double knockout MEFs.
PY - 2002
Y1 - 2002
N2 - We have purified a human histone H4 lysine 20 methyltransferase and cloned the encoding gene, PR/SET07. A mutation in Drosophila pr-set7 is lethal: second instar larval death coincides with the loss of H4 lysine 20 methylation, indicating a fundamental role for PR-Set7 in development. Transcriptionally competent regions lack H4 lysine 20 methylation, but the modification coincided with condensed chromosomal regions on polytene chromosomes, including chromocenter and euchromatic arms. The Drosophila male X chromosome, which is hyperacetylated at H4 lysine 16, has significantly decreased levels of lysine 20 methylation compared to that of females. In vitro, methylation of lysine 20 and acetylation of lysine 16 on the H4 tail are competitive. Taken together, these results support the hypothesis that methylation of H4 lysine 20 maintains silent chromatin, in part, by precluding neighboring acetylation on the H4 tail.
AB - We have purified a human histone H4 lysine 20 methyltransferase and cloned the encoding gene, PR/SET07. A mutation in Drosophila pr-set7 is lethal: second instar larval death coincides with the loss of H4 lysine 20 methylation, indicating a fundamental role for PR-Set7 in development. Transcriptionally competent regions lack H4 lysine 20 methylation, but the modification coincided with condensed chromosomal regions on polytene chromosomes, including chromocenter and euchromatic arms. The Drosophila male X chromosome, which is hyperacetylated at H4 lysine 16, has significantly decreased levels of lysine 20 methylation compared to that of females. In vitro, methylation of lysine 20 and acetylation of lysine 16 on the H4 tail are competitive. Taken together, these results support the hypothesis that methylation of H4 lysine 20 maintains silent chromatin, in part, by precluding neighboring acetylation on the H4 tail.
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U2 - 10.1016/S1097-2765(02)00548-8
DO - 10.1016/S1097-2765(02)00548-8
M3 - Article
C2 - 12086618
AN - SCOPUS:18444392703
SN - 1097-2765
VL - 9
SP - 1201
EP - 1213
JO - Molecular cell
JF - Molecular cell
IS - 6
ER -