Predicting CRISPR/Cas9-Induced Mutations for Precise Genome Editing

Kutubuddin A. Molla; Yinong Yang

doi:10.1016/j.tibtech.2019.08.002

Predicting CRISPR/Cas9-Induced Mutations for Precise Genome Editing

Kutubuddin A. Molla, Yinong Yang

Research output: Contribution to journal › Short survey › peer-review

48 Scopus citations

Abstract

SpCas9 creates blunt end cuts in the genome and generates random and unpredictable mutations through error-prone repair systems. However, a growing body of recent evidence points instead to Cas9-induced staggered end generation, nonrandomness of mutations, and the predictability of editing outcomes using machine learning models.

Original language	English (US)
Pages (from-to)	136-141
Number of pages	6
Journal	Trends in Biotechnology
Volume	38
Issue number	2
DOIs	https://doi.org/10.1016/j.tibtech.2019.08.002
State	Published - Feb 2020

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering

Access to Document

10.1016/j.tibtech.2019.08.002

Cite this

@article{201eaeea119043fabb44c5a53aa61a0d,

title = "Predicting CRISPR/Cas9-Induced Mutations for Precise Genome Editing",

abstract = "SpCas9 creates blunt end cuts in the genome and generates random and unpredictable mutations through error-prone repair systems. However, a growing body of recent evidence points instead to Cas9-induced staggered end generation, nonrandomness of mutations, and the predictability of editing outcomes using machine learning models.",

author = "Molla, {Kutubuddin A.} and Yinong Yang",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2020",

month = feb,

doi = "10.1016/j.tibtech.2019.08.002",

language = "English (US)",

volume = "38",

pages = "136--141",

journal = "Trends in Biotechnology",

issn = "0167-7799",

publisher = "Elsevier Ltd",

number = "2",

}

TY - JOUR

T1 - Predicting CRISPR/Cas9-Induced Mutations for Precise Genome Editing

AU - Molla, Kutubuddin A.

AU - Yang, Yinong

PY - 2020/2

Y1 - 2020/2

N2 - SpCas9 creates blunt end cuts in the genome and generates random and unpredictable mutations through error-prone repair systems. However, a growing body of recent evidence points instead to Cas9-induced staggered end generation, nonrandomness of mutations, and the predictability of editing outcomes using machine learning models.

AB - SpCas9 creates blunt end cuts in the genome and generates random and unpredictable mutations through error-prone repair systems. However, a growing body of recent evidence points instead to Cas9-induced staggered end generation, nonrandomness of mutations, and the predictability of editing outcomes using machine learning models.

UR - http://www.scopus.com/inward/record.url?scp=85072085765&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072085765&partnerID=8YFLogxK

U2 - 10.1016/j.tibtech.2019.08.002

DO - 10.1016/j.tibtech.2019.08.002

M3 - Short survey

C2 - 31526571

AN - SCOPUS:85072085765

SN - 0167-7799

VL - 38

SP - 136

EP - 141

JO - Trends in Biotechnology

JF - Trends in Biotechnology

IS - 2

ER -

Predicting CRISPR/Cas9-Induced Mutations for Precise Genome Editing

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this