PREPARATION, PURIFICATION AND IDENTIFICATION OF 10‐OXO‐TRANS‐8‐DECENOIC ACID FROM THE CULTIVATED MUSHROOM, AGARICUS BISPORUS

JENG‐LEUN ‐L MAU; ROBERT B. BEELMAN; GREGORY R. ZIEGLER

doi:10.1111/j.1745-4514.1992.tb00460.x

PREPARATION, PURIFICATION AND IDENTIFICATION OF 10‐OXO‐TRANS‐8‐DECENOIC ACID FROM THE CULTIVATED MUSHROOM, AGARICUS BISPORUS

JENG‐LEUN ‐L MAU, ROBERT B. BEELMAN, GREGORY R. ZIEGLER

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

ABSTRACT A procedure was developed to isolate 10‐oxo‐trans‐8‐decenoic acid (ODA) from mushrooms. ODA was produced by homogenizing mushrooms in phosphate buffer with added linoleic acid, extracted from the supernatant after centrifugation, and purified using column and thin‐layer chromatography. The purified compound was then characterized using ultraviolet, infrared and mass spectrometry, and nuclear magnetic resonance spectroscopy. The purified compound, containing 97.5% ODA, was a white, waxy solid with a pK_a of 4.68. ODA was soluble in acetone, chloroform, ethanol, ethyl ether, methanol, methylene chloride and water, and slightly soluble in pentane, hexane, heptane and benzene. The TBA test was found to be a viable method for the quantification of ODA.

Original language	English (US)
Pages (from-to)	371-388
Number of pages	18
Journal	Journal of Food Biochemistry
Volume	16
Issue number	6
DOIs	https://doi.org/10.1111/j.1745-4514.1992.tb00460.x
State	Published - Dec 1992

All Science Journal Classification (ASJC) codes

Food Science
Biophysics
Pharmacology
Cell Biology

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title = "PREPARATION, PURIFICATION AND IDENTIFICATION OF 10‐OXO‐TRANS‐8‐DECENOIC ACID FROM THE CULTIVATED MUSHROOM, AGARICUS BISPORUS",

abstract = "ABSTRACT A procedure was developed to isolate 10‐oxo‐trans‐8‐decenoic acid (ODA) from mushrooms. ODA was produced by homogenizing mushrooms in phosphate buffer with added linoleic acid, extracted from the supernatant after centrifugation, and purified using column and thin‐layer chromatography. The purified compound was then characterized using ultraviolet, infrared and mass spectrometry, and nuclear magnetic resonance spectroscopy. The purified compound, containing 97.5% ODA, was a white, waxy solid with a pKa of 4.68. ODA was soluble in acetone, chloroform, ethanol, ethyl ether, methanol, methylene chloride and water, and slightly soluble in pentane, hexane, heptane and benzene. The TBA test was found to be a viable method for the quantification of ODA.",

author = "MAU, {JENG‐LEUN ‐L} and BEELMAN, {ROBERT B.} and ZIEGLER, {GREGORY R.}",

year = "1992",

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doi = "10.1111/j.1745-4514.1992.tb00460.x",

language = "English (US)",

volume = "16",

pages = "371--388",

journal = "Journal of Food Biochemistry",

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publisher = "John Wiley and Sons Inc.",

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AU - MAU, JENG‐LEUN ‐L

AU - BEELMAN, ROBERT B.

AU - ZIEGLER, GREGORY R.

PY - 1992/12

Y1 - 1992/12

N2 - ABSTRACT A procedure was developed to isolate 10‐oxo‐trans‐8‐decenoic acid (ODA) from mushrooms. ODA was produced by homogenizing mushrooms in phosphate buffer with added linoleic acid, extracted from the supernatant after centrifugation, and purified using column and thin‐layer chromatography. The purified compound was then characterized using ultraviolet, infrared and mass spectrometry, and nuclear magnetic resonance spectroscopy. The purified compound, containing 97.5% ODA, was a white, waxy solid with a pKa of 4.68. ODA was soluble in acetone, chloroform, ethanol, ethyl ether, methanol, methylene chloride and water, and slightly soluble in pentane, hexane, heptane and benzene. The TBA test was found to be a viable method for the quantification of ODA.

AB - ABSTRACT A procedure was developed to isolate 10‐oxo‐trans‐8‐decenoic acid (ODA) from mushrooms. ODA was produced by homogenizing mushrooms in phosphate buffer with added linoleic acid, extracted from the supernatant after centrifugation, and purified using column and thin‐layer chromatography. The purified compound was then characterized using ultraviolet, infrared and mass spectrometry, and nuclear magnetic resonance spectroscopy. The purified compound, containing 97.5% ODA, was a white, waxy solid with a pKa of 4.68. ODA was soluble in acetone, chloroform, ethanol, ethyl ether, methanol, methylene chloride and water, and slightly soluble in pentane, hexane, heptane and benzene. The TBA test was found to be a viable method for the quantification of ODA.

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PREPARATION, PURIFICATION AND IDENTIFICATION OF 10‐OXO‐TRANS‐8‐DECENOIC ACID FROM THE CULTIVATED MUSHROOM, AGARICUS BISPORUS

Abstract

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