TY - JOUR
T1 - Prevalence and changes in hepatitis C virus genotypes among multitransfused persons with hemophilia
AU - Eyster, M. Elaine
AU - Sherman, Kenneth E.
AU - Goedert, James J.
AU - Katsoulidou, Antigoni
AU - Hatzakis, Angeles
N1 - Funding Information:
Received 30 June 1998; revised 21 December 1998. Presented in part: 35th annual meeting of the American Society of Hematology, Orlando, Florida, December 1996 (abstract 2093). Informed consent was obtained and the experimentation guidelines of the Human Subjects Protection Office of the Milton S. Hershey Medical Center were followed in the conduct of this research. Financial Support: Alice Livingston Trout Trust Fund, Brandywine Valley Hemophilia Foundation, and National Cancer Institute contract NO1-CP-33002 with Research Triangle Institute. Reprints or correspondence: Dr. M. Elaine Eyster, The Pennsylvania State University College of Medicine, Division of Hematology/Oncology HO46, P. O. Box 850, Hershey, PA 17033 ([email protected]).
PY - 1999
Y1 - 1999
N2 - The purpose of this study was to determine hepatitis C virus (HCV) genotypes and their relationship to HCV RNA levels over time in a cohort of multitransfused hemophiliacs. Following reverse transcription and polymerase chain reaction amplification of HCV RNA, the product DNAs were genotyped by using the line probe assay. HCV RNA was quantified by the branched-chain DNA assay. Genotyping was done on 109 serum samples from 32 subjects. Genotype 3a had the highest prevalence (41%), followed by genotypes 1a (31%) and 1b (13%). Changes in genotypes were observed in 18 (58%) of the subjects >3-15 years of age. Changes were more common in human immunodeficiency virus (HIV)- positive subjects (13/17) than in HIV-negative subjects (5/15) (P = .014). HCV RNA increased 30-fold in HIV-positive subjects whose genotypes changed. Consensus nucleotide sequencing confirmed genotype changes in 2 patients. We conclude that genotype changes are common in hemophiliacs with chronic HCV, particularly in those who are coinfected with HIV.
AB - The purpose of this study was to determine hepatitis C virus (HCV) genotypes and their relationship to HCV RNA levels over time in a cohort of multitransfused hemophiliacs. Following reverse transcription and polymerase chain reaction amplification of HCV RNA, the product DNAs were genotyped by using the line probe assay. HCV RNA was quantified by the branched-chain DNA assay. Genotyping was done on 109 serum samples from 32 subjects. Genotype 3a had the highest prevalence (41%), followed by genotypes 1a (31%) and 1b (13%). Changes in genotypes were observed in 18 (58%) of the subjects >3-15 years of age. Changes were more common in human immunodeficiency virus (HIV)- positive subjects (13/17) than in HIV-negative subjects (5/15) (P = .014). HCV RNA increased 30-fold in HIV-positive subjects whose genotypes changed. Consensus nucleotide sequencing confirmed genotype changes in 2 patients. We conclude that genotype changes are common in hemophiliacs with chronic HCV, particularly in those who are coinfected with HIV.
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U2 - 10.1086/314708
DO - 10.1086/314708
M3 - Article
C2 - 10191205
AN - SCOPUS:0033009317
SN - 0022-1899
VL - 179
SP - 1062
EP - 1069
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 5
ER -
