To determine whether primary cilia act as mechanosensors in bone, intracellular calcium release and prostaglandin E2 release were examined. The existence of primary cilia in bone tissue was confirmed using immunoflourescent detection of acetylated α-tubulin. PGE2 was quantifued using a commercial ELISA kiet and normalized to total protein. Real-time intracellular levels of calcium were quantified.
|Number of pages
|MCB Molecular and Cellular Biomechanics
|Published - Dec 1 2006
All Science Journal Classification (ASJC) codes
- Molecular Medicine
- Molecular Biology
- Cell Biology