Primary cilia mediate PGE2 release in MC3T3-E1 osteoblasts

A. M.D. Malone; Charles T. Anderson; P. Tummala; T. Stearns; C. R. Jacobs

Primary cilia mediate PGE₂ release in MC3T3-E1 osteoblasts

A. M.D. Malone, Charles T. Anderson, P. Tummala, T. Stearns, C. R. Jacobs

Research output: Contribution to journal › Article › peer-review

Abstract

To determine whether primary cilia act as mechanosensors in bone, intracellular calcium release and prostaglandin E₂ release were examined. The existence of primary cilia in bone tissue was confirmed using immunoflourescent detection of acetylated α-tubulin. PGE₂ was quantifued using a commercial ELISA kiet and normalized to total protein. Real-time intracellular levels of calcium were quantified.

Original language	English (US)
Pages (from-to)	207-208
Number of pages	2
Journal	MCB Molecular and Cellular Biomechanics
Volume	3
Issue number	4
State	Published - Dec 1 2006

All Science Journal Classification (ASJC) codes

Biophysics
Molecular Medicine
Molecular Biology
Cell Biology

Cite this

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title = "Primary cilia mediate PGE2 release in MC3T3-E1 osteoblasts",

abstract = "To determine whether primary cilia act as mechanosensors in bone, intracellular calcium release and prostaglandin E2 release were examined. The existence of primary cilia in bone tissue was confirmed using immunoflourescent detection of acetylated α-tubulin. PGE2 was quantifued using a commercial ELISA kiet and normalized to total protein. Real-time intracellular levels of calcium were quantified.",

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AU - Malone, A. M.D.

AU - Anderson, Charles T.

AU - Tummala, P.

AU - Stearns, T.

AU - Jacobs, C. R.

PY - 2006/12/1

Y1 - 2006/12/1

N2 - To determine whether primary cilia act as mechanosensors in bone, intracellular calcium release and prostaglandin E2 release were examined. The existence of primary cilia in bone tissue was confirmed using immunoflourescent detection of acetylated α-tubulin. PGE2 was quantifued using a commercial ELISA kiet and normalized to total protein. Real-time intracellular levels of calcium were quantified.

AB - To determine whether primary cilia act as mechanosensors in bone, intracellular calcium release and prostaglandin E2 release were examined. The existence of primary cilia in bone tissue was confirmed using immunoflourescent detection of acetylated α-tubulin. PGE2 was quantifued using a commercial ELISA kiet and normalized to total protein. Real-time intracellular levels of calcium were quantified.

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Primary cilia mediate PGE₂ release in MC3T3-E1 osteoblasts

Abstract

All Science Journal Classification (ASJC) codes

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