TY - JOUR
T1 - Primer-free aptamer selection using a random DNA library.
AU - Pan, Weihua
AU - Clawson, Gary A.
PY - 2010
Y1 - 2010
N2 - Aptamers are high-affinity oligonucleotides which can be selected from large random libraries by systematic evolution of ligands by exponential enrichment (SELEX) protocols, with affinities and specificities comparable or better than antibodies. The SELEX protocols comprise multiple rounds of selection, each of which require regeneration of bound ligands, which in turn require fixed primer sequences flanking the random library regions. These fixed primer sequences can interfere (with false positives and negatives) with the selection process. Here we present a primer-free protocol using a random DNA library.
AB - Aptamers are high-affinity oligonucleotides which can be selected from large random libraries by systematic evolution of ligands by exponential enrichment (SELEX) protocols, with affinities and specificities comparable or better than antibodies. The SELEX protocols comprise multiple rounds of selection, each of which require regeneration of bound ligands, which in turn require fixed primer sequences flanking the random library regions. These fixed primer sequences can interfere (with false positives and negatives) with the selection process. Here we present a primer-free protocol using a random DNA library.
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U2 - 10.1007/978-1-60761-657-3_24
DO - 10.1007/978-1-60761-657-3_24
M3 - Article
C2 - 20387162
AN - SCOPUS:77954057161
SN - 1064-3745
VL - 629
SP - 369
EP - 385
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -