Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors

Chunfeng Zhang; Justin Ingram; Steven Schiff; Jian Xu; Min Xiao

doi:10.1117/12.876341

Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors

Chunfeng Zhang, Justin Ingram, Steven Schiff, Jian Xu, Min Xiao

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

5 Scopus citations

Abstract

We developed ratiometric optical oxygen sensors to probe the oxygen consumption during epileptic events in rat brain slices. The oxygen sensors consist of the sensing part of phosphorescence dyes (Platinum (II) octaethylporphine ketone) and reference part of nanocystal quantum dots (NQDs) embedded in polymer blends, with pre-designed excitation through fluorescence resonance energy transfer (FRET) from NQDs to the oxygen sensitive dyes (OSDs). The ratiometric FRET sensors with fast temporal response and excellent bio-compatibility are suitable for real time quantitative dissolved oxygen (D.O.) probes in biological microenvironment. Coating the sensors onto the micro-pipettes, we performed simultaneous oxygen probes at pyramidal and oriens layers in rat hippocampal CA1. Different spatiotemporal patterns with maximum D.O. decreases of 9.9±1.1 mg/L and 4.9±0.7 mg/L during seizure events were observed in pyramidal and oriens layers, respectively.

Original language	English (US)
Title of host publication	Quantum Dots and Nanostructures
Subtitle of host publication	Synthesis, Characterization, and Modeling VIII
Volume	7947
DOIs	https://doi.org/10.1117/12.876341
State	Published - Apr 27 2011
Event	Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII - San Francisco, CA, United States Duration: Jan 25 2011 → Jan 26 2011

Other

Other	Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII
Country/Territory	United States
City	San Francisco, CA
Period	1/25/11 → 1/26/11

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.876341

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title = "Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors",

abstract = "We developed ratiometric optical oxygen sensors to probe the oxygen consumption during epileptic events in rat brain slices. The oxygen sensors consist of the sensing part of phosphorescence dyes (Platinum (II) octaethylporphine ketone) and reference part of nanocystal quantum dots (NQDs) embedded in polymer blends, with pre-designed excitation through fluorescence resonance energy transfer (FRET) from NQDs to the oxygen sensitive dyes (OSDs). The ratiometric FRET sensors with fast temporal response and excellent bio-compatibility are suitable for real time quantitative dissolved oxygen (D.O.) probes in biological microenvironment. Coating the sensors onto the micro-pipettes, we performed simultaneous oxygen probes at pyramidal and oriens layers in rat hippocampal CA1. Different spatiotemporal patterns with maximum D.O. decreases of 9.9±1.1 mg/L and 4.9±0.7 mg/L during seizure events were observed in pyramidal and oriens layers, respectively.",

author = "Chunfeng Zhang and Justin Ingram and Steven Schiff and Jian Xu and Min Xiao",

year = "2011",

month = apr,

day = "27",

doi = "10.1117/12.876341",

language = "English (US)",

isbn = "9780819484840",

volume = "7947",

booktitle = "Quantum Dots and Nanostructures",

note = "Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII ; Conference date: 25-01-2011 Through 26-01-2011",

}

Zhang, C, Ingram, J, Schiff, S, Xu, J & Xiao, M 2011, Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors. in Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII. vol. 7947, 79470B, Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII, San Francisco, CA, United States, 1/25/11. https://doi.org/10.1117/12.876341

TY - GEN

T1 - Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors

AU - Zhang, Chunfeng

AU - Ingram, Justin

AU - Schiff, Steven

AU - Xu, Jian

AU - Xiao, Min

PY - 2011/4/27

Y1 - 2011/4/27

N2 - We developed ratiometric optical oxygen sensors to probe the oxygen consumption during epileptic events in rat brain slices. The oxygen sensors consist of the sensing part of phosphorescence dyes (Platinum (II) octaethylporphine ketone) and reference part of nanocystal quantum dots (NQDs) embedded in polymer blends, with pre-designed excitation through fluorescence resonance energy transfer (FRET) from NQDs to the oxygen sensitive dyes (OSDs). The ratiometric FRET sensors with fast temporal response and excellent bio-compatibility are suitable for real time quantitative dissolved oxygen (D.O.) probes in biological microenvironment. Coating the sensors onto the micro-pipettes, we performed simultaneous oxygen probes at pyramidal and oriens layers in rat hippocampal CA1. Different spatiotemporal patterns with maximum D.O. decreases of 9.9±1.1 mg/L and 4.9±0.7 mg/L during seizure events were observed in pyramidal and oriens layers, respectively.

AB - We developed ratiometric optical oxygen sensors to probe the oxygen consumption during epileptic events in rat brain slices. The oxygen sensors consist of the sensing part of phosphorescence dyes (Platinum (II) octaethylporphine ketone) and reference part of nanocystal quantum dots (NQDs) embedded in polymer blends, with pre-designed excitation through fluorescence resonance energy transfer (FRET) from NQDs to the oxygen sensitive dyes (OSDs). The ratiometric FRET sensors with fast temporal response and excellent bio-compatibility are suitable for real time quantitative dissolved oxygen (D.O.) probes in biological microenvironment. Coating the sensors onto the micro-pipettes, we performed simultaneous oxygen probes at pyramidal and oriens layers in rat hippocampal CA1. Different spatiotemporal patterns with maximum D.O. decreases of 9.9±1.1 mg/L and 4.9±0.7 mg/L during seizure events were observed in pyramidal and oriens layers, respectively.

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M3 - Conference contribution

AN - SCOPUS:79955067232

SN - 9780819484840

VL - 7947

BT - Quantum Dots and Nanostructures

T2 - Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII

Y2 - 25 January 2011 through 26 January 2011

ER -

Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors

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