TY - JOUR
T1 - Probing pore structure of virus filters using scanning electron microscopy with gold nanoparticles
AU - Nazem-Bokaee, Hadi
AU - Fallahianbijan, Fatemeh
AU - Chen, Dayue
AU - O'Donnell, Sean Michael
AU - Carbrello, Christina
AU - Giglia, Sal
AU - Bell, David
AU - Zydney, Andrew L.
N1 - Funding Information:
The authors would like to acknowledge the Lilly Research Award Program (LRAP) from Eli Lilly and MilliporeSigma for their financial support of this work. Significant assistance with the SEM sample preparation and image analysis was provided by the Huck Life Sciences Institute and Materials Characterization Laboratory (MCL) at The Pennsylvania State University.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/4/15
Y1 - 2018/4/15
N2 - The performance characteristics of virus filtration membranes are strongly influenced by the underlying pore size and morphology of the membrane, but the available characterization techniques provide only limited information on these properties. A new methodology was developed for probing the internal pore size and structure of different virus filtration membranes by direct visualization of captured gold nanoparticles using scanning electron microscopy (SEM). SEM images were obtained using both hollow fiber (Planova™ 20 N and BioEX) and flat sheet (Viresolve® Pro and Ultipor® DV20) membranes after challenging with 20, 40, and / or 100 nm gold nanoparticles. The images were used to identify the location of individual nanoparticles and to calculate the nanoparticle distribution through the depth of the filter (using ImageJ software). The results clearly demonstrate the very different pore structures within these filters and their impact on nanoparticle capture, providing important insights into the performance characteristics of these virus filtration membranes.
AB - The performance characteristics of virus filtration membranes are strongly influenced by the underlying pore size and morphology of the membrane, but the available characterization techniques provide only limited information on these properties. A new methodology was developed for probing the internal pore size and structure of different virus filtration membranes by direct visualization of captured gold nanoparticles using scanning electron microscopy (SEM). SEM images were obtained using both hollow fiber (Planova™ 20 N and BioEX) and flat sheet (Viresolve® Pro and Ultipor® DV20) membranes after challenging with 20, 40, and / or 100 nm gold nanoparticles. The images were used to identify the location of individual nanoparticles and to calculate the nanoparticle distribution through the depth of the filter (using ImageJ software). The results clearly demonstrate the very different pore structures within these filters and their impact on nanoparticle capture, providing important insights into the performance characteristics of these virus filtration membranes.
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U2 - 10.1016/j.memsci.2018.01.069
DO - 10.1016/j.memsci.2018.01.069
M3 - Article
AN - SCOPUS:85041524704
SN - 0376-7388
VL - 552
SP - 144
EP - 152
JO - Journal of Membrane Science
JF - Journal of Membrane Science
ER -