Probing the Functional Role of Phenylalanine-31 of Escherichia coli Dihydrofolate Reductase by Site-Directed Mutagenesis

Keyphrases

• Functional Role 100%
• Site-directed mutagenesis 100%
• Phenylalanine 100%
• Escherichia Coli Dihydrofolate Reductase 100%
• Mutant Enzyme 100%
• Product Complexity 66%
• Tetrahydrofolate 66%
• Fold Increase 33%
• Rate-limiting Step 33%
• Amino Acid Substitution 33%
• Hydrophobic Pocket 33%
• Conserved Residues 33%
• Rate Limiting 33%
• Ternary Complex 33%
• Ultrafast Dissociation 33%
• Kinetic Behavior 33%
• Hydride Transfer 33%
• Opposing Effects 33%
• Dissociation Rate 33%
• Dihydrofolate 33%
• Quantitative Effect 33%

Biochemistry, Genetics and Molecular Biology

• Escherichia coli 100%
• Site-Directed Mutagenesis 100%
• Enzyme 100%
• Dihydrofolate Reductase 100%
• Phenylalanine 100%
• Wild Type 50%
• Tetrahydrofolic Acid 50%
• Amino Acid Substitution 25%
• Ternary Complex 25%