Production of anti‐H‐2 antibodies in thymectomized mice

J. Klein, S. Livnat, V. Hauptfeld, L. Jeřábek, I. Weissman

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Neonatally and adult thymectomized (Tx) mice of strains C57BL/10Sn and A. BY (H‐2b) were immunized at the age of 5 – 8 weeks with lethally irradiated spleen and lymph node cells from adult B 10. A and A (H‐2a) donors, respectively. The titer of H‐2 hemagglutinins and cytotoxins was determined at different intervals after immunization. Both untreated antibodies and antibodies reduced with 2‐mercaptoethanol (2‐Me) were tested. The non‐Tx control mice responded with production of both 2‐Me‐sensitive and 2‐Me‐resistant H‐2 antibodies. In the Tx mice, only 2‐Me‐sensitive H‐2 antibodies were detected. Thus, the anti‐H‐2 IgM response appears to be thymus‐independent and the anti‐H‐2 IgG response thymus‐dependent.

Original languageEnglish (US)
Pages (from-to)41-44
Number of pages4
JournalEuropean Journal of Immunology
Volume4
Issue number1
DOIs
StatePublished - Jan 1974

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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