Production of valinomycin, an insecticidal antibiotic, by streptomyces griseus var. flexipertum var. nov

Rod M. Heisey; Saroj K. Mishra; James E. Keller; James R. Miller; Alan R. Putnam; Jamin Huang; Themistocles D.J. D'Silva

doi:10.1021/jf00084a039

Production of valinomycin, an insecticidal antibiotic, by streptomyces griseus var. flexipertum var. nov

Rod M. Heisey, Saroj K. Mishra, James E. Keller, James R. Miller, Alan R. Putnam, Jamin Huang, Themistocles D.J. D'Silva

Penn State Schuylkill

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A screening program to discover microorganisms that produce novel pesticides has yielded a new streptomycete strain that produces valinomycin, an insecticidal and acaricidal antibiotic. Bioassays of the crude culture broth produced by this strain demonstrated an LC₅₀ to mosquito larvae of 10^-3-10^-4 dilution. Bioassays of the purified insecticide yielded LC₅₀ values of 2-3 pm for mosquito larvae, 3 ppm for two-spotted spider mites, and 35 ppm for Mexican bean beetle larvae. Taxonomic studies indicated the valinomycin-producing microorganism was an atypical variant of Streptomyces griseus, which is hereby named var. flexipertum var. nov. Morphology and physiology of the new microorganism and production, isolation, and identification of the insecticidal metabolite are described.

Original language	English (US)
Pages (from-to)	1283-1286
Number of pages	4
Journal	Journal of agricultural and food chemistry
Volume	36
Issue number	6
DOIs	https://doi.org/10.1021/jf00084a039
State	Published - Nov 1 1988

All Science Journal Classification (ASJC) codes

General Chemistry
General Agricultural and Biological Sciences

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10.1021/jf00084a039

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title = "Production of valinomycin, an insecticidal antibiotic, by streptomyces griseus var. flexipertum var. nov",

abstract = "A screening program to discover microorganisms that produce novel pesticides has yielded a new streptomycete strain that produces valinomycin, an insecticidal and acaricidal antibiotic. Bioassays of the crude culture broth produced by this strain demonstrated an LC50 to mosquito larvae of 10-3-10-4 dilution. Bioassays of the purified insecticide yielded LC50 values of 2-3 pm for mosquito larvae, 3 ppm for two-spotted spider mites, and 35 ppm for Mexican bean beetle larvae. Taxonomic studies indicated the valinomycin-producing microorganism was an atypical variant of Streptomyces griseus, which is hereby named var. flexipertum var. nov. Morphology and physiology of the new microorganism and production, isolation, and identification of the insecticidal metabolite are described.",

author = "Heisey, {Rod M.} and Mishra, {Saroj K.} and Keller, {James E.} and Miller, {James R.} and Putnam, {Alan R.} and Jamin Huang and D'Silva, {Themistocles D.J.}",

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T1 - Production of valinomycin, an insecticidal antibiotic, by streptomyces griseus var. flexipertum var. nov

AU - Heisey, Rod M.

AU - Mishra, Saroj K.

AU - Keller, James E.

AU - Miller, James R.

AU - Putnam, Alan R.

AU - Huang, Jamin

AU - D'Silva, Themistocles D.J.

PY - 1988/11/1

Y1 - 1988/11/1

N2 - A screening program to discover microorganisms that produce novel pesticides has yielded a new streptomycete strain that produces valinomycin, an insecticidal and acaricidal antibiotic. Bioassays of the crude culture broth produced by this strain demonstrated an LC50 to mosquito larvae of 10-3-10-4 dilution. Bioassays of the purified insecticide yielded LC50 values of 2-3 pm for mosquito larvae, 3 ppm for two-spotted spider mites, and 35 ppm for Mexican bean beetle larvae. Taxonomic studies indicated the valinomycin-producing microorganism was an atypical variant of Streptomyces griseus, which is hereby named var. flexipertum var. nov. Morphology and physiology of the new microorganism and production, isolation, and identification of the insecticidal metabolite are described.

AB - A screening program to discover microorganisms that produce novel pesticides has yielded a new streptomycete strain that produces valinomycin, an insecticidal and acaricidal antibiotic. Bioassays of the crude culture broth produced by this strain demonstrated an LC50 to mosquito larvae of 10-3-10-4 dilution. Bioassays of the purified insecticide yielded LC50 values of 2-3 pm for mosquito larvae, 3 ppm for two-spotted spider mites, and 35 ppm for Mexican bean beetle larvae. Taxonomic studies indicated the valinomycin-producing microorganism was an atypical variant of Streptomyces griseus, which is hereby named var. flexipertum var. nov. Morphology and physiology of the new microorganism and production, isolation, and identification of the insecticidal metabolite are described.

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Production of valinomycin, an insecticidal antibiotic, by streptomyces griseus var. flexipertum var. nov

Abstract

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