TY - JOUR
T1 - Propagation, infection, and neutralization of authentic HPV16 virus
AU - McLaughlin-Drubin, Margaret E.
AU - Christensen, Neil D.
AU - Meyers, Craig
N1 - Funding Information:
We thank members of the Meyers' laboratory for many helpful discussions, Dr. M. Dürst for the HPV16(114/B) and (114/K) clones, and Lyn Budgeon for histological assistance. This work was supported by NIH Training grant 5 T32 60395-10 (M.M.-D.) and grant RO1 CA79006 (C.M.).
PY - 2004/5/1
Y1 - 2004/5/1
N2 - Despite the prevalence of HPV16 in invasive cervical cancers, an in vitro system capable of producing infectious HPV16 is lacking. The organotypic (raft) culture system has allowed for the study of the entire differentiation-dependent life cycle of human papillomaviruses (HPVs). However, the use of this system with the prototype HPV16-containing cell line, W12, has failed to yield infectious virus. Our laboratory has introduced clinically derived HPV16(114/B) genomic DNA into primary keratinocytes, where it subsequently recircularized and maintained episomally at 50-100 copies per cell. Virion morphogenesis occurred after epithelial stratification and differentiation in raft culture. HPV16 virions were isolated that were able to infect keratinocytes in vitro. Infection was neutralized by monoclonal antibodies raised against HPV16 but not by monoclonal antibodies known to neutralize other HPV types.
AB - Despite the prevalence of HPV16 in invasive cervical cancers, an in vitro system capable of producing infectious HPV16 is lacking. The organotypic (raft) culture system has allowed for the study of the entire differentiation-dependent life cycle of human papillomaviruses (HPVs). However, the use of this system with the prototype HPV16-containing cell line, W12, has failed to yield infectious virus. Our laboratory has introduced clinically derived HPV16(114/B) genomic DNA into primary keratinocytes, where it subsequently recircularized and maintained episomally at 50-100 copies per cell. Virion morphogenesis occurred after epithelial stratification and differentiation in raft culture. HPV16 virions were isolated that were able to infect keratinocytes in vitro. Infection was neutralized by monoclonal antibodies raised against HPV16 but not by monoclonal antibodies known to neutralize other HPV types.
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U2 - 10.1016/j.virol.2004.02.011
DO - 10.1016/j.virol.2004.02.011
M3 - Article
C2 - 15110519
AN - SCOPUS:1942489261
SN - 0042-6822
VL - 322
SP - 213
EP - 219
JO - Virology
JF - Virology
IS - 2
ER -
