Prophages in Listeria monocytogenes contain single-nucleotide polymorphisms that differentiate outbreak clones within epidemic clones

Yi Chen; Stephen J. Knabel

doi:10.1128/JCM.01873-07

Prophages in Listeria monocytogenes contain single-nucleotide polymorphisms that differentiate outbreak clones within epidemic clones

Yi Chen, Stephen J. Knabel

Food Science

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Abstract

A fragment end ligation-mediated PCR strategy was used to analyze the AscI pulsed-field gel electrophoresis patterns of Listeria monocytogenes epidemic clone II (ECII), which led to the identification of single-nucleotide polymorphisms (SNPs) in prophage regions that differentiated the two ECII outbreak clones. SNPs in prophages that differentiated the outbreak clones of ECIII and -IV were also identified.

Original language	English (US)
Pages (from-to)	1478-1484
Number of pages	7
Journal	Journal of clinical microbiology
Volume	46
Issue number	4
DOIs	https://doi.org/10.1128/JCM.01873-07
State	Published - Apr 2008

All Science Journal Classification (ASJC) codes

Microbiology (medical)

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10.1128/JCM.01873-07

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abstract = "A fragment end ligation-mediated PCR strategy was used to analyze the AscI pulsed-field gel electrophoresis patterns of Listeria monocytogenes epidemic clone II (ECII), which led to the identification of single-nucleotide polymorphisms (SNPs) in prophage regions that differentiated the two ECII outbreak clones. SNPs in prophages that differentiated the outbreak clones of ECIII and -IV were also identified.",

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AB - A fragment end ligation-mediated PCR strategy was used to analyze the AscI pulsed-field gel electrophoresis patterns of Listeria monocytogenes epidemic clone II (ECII), which led to the identification of single-nucleotide polymorphisms (SNPs) in prophage regions that differentiated the two ECII outbreak clones. SNPs in prophages that differentiated the outbreak clones of ECIII and -IV were also identified.

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Prophages in Listeria monocytogenes contain single-nucleotide polymorphisms that differentiate outbreak clones within epidemic clones

Abstract

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