Protection of hematopoietic cells against combined O6-benzylguanine and chloroethylnitrosourea treatment by mutant forms of O6-methylguanine DNA methyltransferase

D. A. Williams; R. Maze; C. Kurpad; A. Pegg; L. C. Erickson

doi:10.1038/sj.bmt.1702367

Protection of hematopoietic cells against combined O⁶-benzylguanine and chloroethylnitrosourea treatment by mutant forms of O⁶-methylguanine DNA methyltransferase

D. A. Williams, R. Maze, C. Kurpad, A. Pegg, L. C. Erickson

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9 Scopus citations

Abstract

This report demonstrates that expression of the P140A O⁶-methylguanine DNA methyl transferase (MGMT) mutant via retrovirus-mediated gene transfer leads to significant, but modest, resistance of cells to both 6-benzylguanine (6-BG) depletion and treatment with 1,3-bis (2-chloroethyl)-1-nitrosourea (BCNU). Expression of the P140A/G156A double mutant appeared to be associated with reduced or unstable protein in hematopoietic cells. Bone Marrow Transplantation (2000) 25, Suppl. 2, S105–S109.

Original language	English (US)
Pages (from-to)	S105-S109
Journal	Bone Marrow Transplantation
Volume	25
DOIs	https://doi.org/10.1038/sj.bmt.1702367
State	Published - May 2000

All Science Journal Classification (ASJC) codes

Hematology
Transplantation

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10.1038/sj.bmt.1702367

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title = "Protection of hematopoietic cells against combined O6-benzylguanine and chloroethylnitrosourea treatment by mutant forms of O6-methylguanine DNA methyltransferase",

abstract = "This report demonstrates that expression of the P140A O6-methylguanine DNA methyl transferase (MGMT) mutant via retrovirus-mediated gene transfer leads to significant, but modest, resistance of cells to both 6-benzylguanine (6-BG) depletion and treatment with 1,3-bis (2-chloroethyl)-1-nitrosourea (BCNU). Expression of the P140A/G156A double mutant appeared to be associated with reduced or unstable protein in hematopoietic cells. Bone Marrow Transplantation (2000) 25, Suppl. 2, S105–S109.",

author = "Williams, {D. A.} and R. Maze and C. Kurpad and A. Pegg and Erickson, {L. C.}",

note = "Funding Information: This work was supported by NCI grants PO1CA 75426 (DAW) and CA 45628-10 (LE). We thank Sharon Smoot and Eva Meun-ier for expert secretarial support.",

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doi = "10.1038/sj.bmt.1702367",

language = "English (US)",

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T1 - Protection of hematopoietic cells against combined O6-benzylguanine and chloroethylnitrosourea treatment by mutant forms of O6-methylguanine DNA methyltransferase

AU - Williams, D. A.

AU - Maze, R.

AU - Kurpad, C.

AU - Pegg, A.

AU - Erickson, L. C.

N1 - Funding Information: This work was supported by NCI grants PO1CA 75426 (DAW) and CA 45628-10 (LE). We thank Sharon Smoot and Eva Meun-ier for expert secretarial support.

PY - 2000/5

Y1 - 2000/5

N2 - This report demonstrates that expression of the P140A O6-methylguanine DNA methyl transferase (MGMT) mutant via retrovirus-mediated gene transfer leads to significant, but modest, resistance of cells to both 6-benzylguanine (6-BG) depletion and treatment with 1,3-bis (2-chloroethyl)-1-nitrosourea (BCNU). Expression of the P140A/G156A double mutant appeared to be associated with reduced or unstable protein in hematopoietic cells. Bone Marrow Transplantation (2000) 25, Suppl. 2, S105–S109.

AB - This report demonstrates that expression of the P140A O6-methylguanine DNA methyl transferase (MGMT) mutant via retrovirus-mediated gene transfer leads to significant, but modest, resistance of cells to both 6-benzylguanine (6-BG) depletion and treatment with 1,3-bis (2-chloroethyl)-1-nitrosourea (BCNU). Expression of the P140A/G156A double mutant appeared to be associated with reduced or unstable protein in hematopoietic cells. Bone Marrow Transplantation (2000) 25, Suppl. 2, S105–S109.

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ER -

Protection of hematopoietic cells against combined O⁶-benzylguanine and chloroethylnitrosourea treatment by mutant forms of O⁶-methylguanine DNA methyltransferase

Abstract

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