Protein engineering of toluene 4-monooxygenase of Pseudomonas mendocina KR1 for synthesizing 4-nitrocatechol from nitrobenzene

Medicine & Life Sciences

• toluene-4-monooxygenase 100%
• 4-nitrocatechol 93%
• Pseudomonas mendocina 90%
• nitrobenzene 80%
• nitrocatechol 73%
• Protein Engineering 64%
• 3-nitrophenol 58%
• Mutagenesis 37%
• 4-cresol 31%
• Toluene 24%
• toluene ortho-monooxygenase 20%
• 3-cresol 16%
• 4-nitrophenol 13%
• Proteins 13%
• Mixed Function Oxygenases 10%
• Enzymes 10%
• Amino Acid Substitution 10%
• Agar 8%
• Catalytic Domain 8%
• Mass Spectrometry 8%
• Libraries 8%
• High Pressure Liquid Chromatography 7%
• Escherichia coli 6%
• Polymerase Chain Reaction 5%

Engineering & Materials Science

• Nitrobenzene 80%
• Toluene 56%
• Nitrophenols 48%
• Proteins 42%
• Mutagenesis 42%
• Oxidation 17%
• Enzymes 15%
• Substrates 14%
• Mass spectrometry 8%
• Escherichia coli 8%
• Amino acids 7%
• Reaction rates 7%
• Substitution reactions 6%
• Cells 6%
• Screening 6%

Chemical Compounds

• 4-Nitrocatechol 77%
• Nitrobenzene 48%
• Toluene 34%
• Protein 19%
• Vmax 14%
• Oxidation Reaction 9%
• Agar 7%
• Plate Like Crystal 5%
• Substitution Reaction 4%
• Amount 2%
• Time 2%
• Acid 2%