There is considerable commercial interest in the preparation of individual whey proteins for food, nutraceutical, and therapeutic applications. Recent developments in membrane filtration have provided exciting new opportunities for large-scale protein fractionation. This manuscript provides an overview of the previous work on protein separations using membrane filtration, including the importance of solution pH and ionic strength to obtain very high resolution separations. Specific data are shown for the separation of hemoglobin from BSA and for the purification of immunoglobulins from a complex feed stream using a gradient diafiltration process. The potential applications of membrane systems for whey protein fractionation are also examined.
All Science Journal Classification (ASJC) codes
- Food Science
- Applied Microbiology and Biotechnology