TY - JOUR
T1 - Protein synthesis in rabbit reticulocytes
T2 - Requirements for Met-tRNAf·40S preinitiation complex formation with AUG-codon and physiological mRNAs
AU - Roy, Reena
AU - Nasrin, Nargis
AU - Ahmad, Mir Firoz
AU - Gupta, Naba K.
PY - 1984/8/16
Y1 - 1984/8/16
N2 - Under standard conditions, in the presence of GTP, highly purified eIF-2 and Co-eIF-2 factor preparations efficiently stimulated AUG-codon dependent but not physiological mRNA-dependent Met-tRNAf binding to 40S ribosomes. Replacement of GTP by a nonhydrolyzable GTP analog, GMP-PNP, in the above system, gave significant stimulation of Met-tRNAf binding to 40S ribosomes dependent on physiological mRNAs. Lower but significant stimulation of Met-tRNAf binding to 40S ribosomes was also observed when GTP was used in the presence of nucleoside 5′-diphosphate kinase (NDK) and ATP. ATP alone in the absence of NDK had no significant effect. This is the first report on the formation of a stable Met-tRNAf·40S initiation complex dependent on physiological mRNAs and the factor requirements for such complex formation.
AB - Under standard conditions, in the presence of GTP, highly purified eIF-2 and Co-eIF-2 factor preparations efficiently stimulated AUG-codon dependent but not physiological mRNA-dependent Met-tRNAf binding to 40S ribosomes. Replacement of GTP by a nonhydrolyzable GTP analog, GMP-PNP, in the above system, gave significant stimulation of Met-tRNAf binding to 40S ribosomes dependent on physiological mRNAs. Lower but significant stimulation of Met-tRNAf binding to 40S ribosomes was also observed when GTP was used in the presence of nucleoside 5′-diphosphate kinase (NDK) and ATP. ATP alone in the absence of NDK had no significant effect. This is the first report on the formation of a stable Met-tRNAf·40S initiation complex dependent on physiological mRNAs and the factor requirements for such complex formation.
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U2 - 10.1016/0006-291X(84)91249-X
DO - 10.1016/0006-291X(84)91249-X
M3 - Article
C2 - 6566570
AN - SCOPUS:0021279803
SN - 0006-291X
VL - 122
SP - 1418
EP - 1425
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -