Protein synthesis in rabbit reticulocytes XIX: EIF-2 promotes dissociation of Met-tRNAf·EIF-1·GTP complex and Met-tRNAf binding to 40S ribosomes

A. Majumdar; R. Roy; A. Das; A. Dasgupta; N. K. Gupta

doi:10.1016/0006-291X(77)91235-9

Protein synthesis in rabbit reticulocytes XIX: EIF-2 promotes dissociation of Met-tRNA_f·EIF-1·GTP complex and Met-tRNA_f binding to 40S ribosomes

A. Majumdar
, R. Roy
, A. Das
, A. Dasgupta
, N. K. Gupta

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The peptide chain initiation factor, EIF-2 has been partially purified from the 0.5 M KCl ribosomal wash. The molecular weight of EIF-2 is approximately 450,000. The purified EIF-2 preparation promotes the dissociation of the ternary complex, Met-tRNA_f·EIF-1·GTP in the presence of Mg⁺⁺ and is also required along with EIF-1 for AUG-directed Met-tRNA_f binding to 40S ribosomes.

Original language	English (US)
Pages (from-to)	161-169
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	78
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(77)91235-9
State	Published - Sep 9 1977

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{d1bf32dd7c0a4f93a7415b2ecb72188e,

title = "Protein synthesis in rabbit reticulocytes XIX: EIF-2 promotes dissociation of Met-tRNAf·EIF-1·GTP complex and Met-tRNAf binding to 40S ribosomes",

abstract = "The peptide chain initiation factor, EIF-2 has been partially purified from the 0.5 M KCl ribosomal wash. The molecular weight of EIF-2 is approximately 450,000. The purified EIF-2 preparation promotes the dissociation of the ternary complex, Met-tRNAf·EIF-1·GTP in the presence of Mg++ and is also required along with EIF-1 for AUG-directed Met-tRNAf binding to 40S ribosomes.",

author = "A. Majumdar and R. Roy and A. Das and A. Dasgupta and Gupta, \{N. K.\}",

note = "Funding Information: Acknowledgements research work was supported by NIH Research Grants GM-18796 The authors thank Mr. Dennis Jurgens for the preparation ribosomes and ribosomal salt wash.",

year = "1977",

month = sep,

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doi = "10.1016/0006-291X(77)91235-9",

language = "English (US)",

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TY - JOUR

T1 - Protein synthesis in rabbit reticulocytes XIX

T2 - EIF-2 promotes dissociation of Met-tRNAf·EIF-1·GTP complex and Met-tRNAf binding to 40S ribosomes

AU - Majumdar, A.

AU - Roy, R.

AU - Das, A.

AU - Dasgupta, A.

AU - Gupta, N. K.

N1 - Funding Information: Acknowledgements research work was supported by NIH Research Grants GM-18796 The authors thank Mr. Dennis Jurgens for the preparation ribosomes and ribosomal salt wash.

PY - 1977/9/9

Y1 - 1977/9/9

N2 - The peptide chain initiation factor, EIF-2 has been partially purified from the 0.5 M KCl ribosomal wash. The molecular weight of EIF-2 is approximately 450,000. The purified EIF-2 preparation promotes the dissociation of the ternary complex, Met-tRNAf·EIF-1·GTP in the presence of Mg++ and is also required along with EIF-1 for AUG-directed Met-tRNAf binding to 40S ribosomes.

AB - The peptide chain initiation factor, EIF-2 has been partially purified from the 0.5 M KCl ribosomal wash. The molecular weight of EIF-2 is approximately 450,000. The purified EIF-2 preparation promotes the dissociation of the ternary complex, Met-tRNAf·EIF-1·GTP in the presence of Mg++ and is also required along with EIF-1 for AUG-directed Met-tRNAf binding to 40S ribosomes.

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UR - https://www.scopus.com/pages/publications/0017753214#tab=citedBy

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AN - SCOPUS:0017753214

SN - 0006-291X

VL - 78

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EP - 169

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Protein synthesis in rabbit reticulocytes XIX: EIF-2 promotes dissociation of Met-tRNA_f·EIF-1·GTP complex and Met-tRNA_f binding to 40S ribosomes

Abstract

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