TY - JOUR
T1 - Protein transport through zwitterionic ultrafiltration membranes
AU - Rohani, Mahsa M.
AU - Zydney, Andrew L.
N1 - Funding Information:
The authors would like to acknowledge Millipore Corporation for donation of the Ultracel™ membranes and the Materials Research Institute at The Pennsylvania State University for assistance with the XPS measurements. This publication was supported by The Pennsylvania State University Materials Research Institute Nanofabrication Lab and the National Science Foundation Cooperative Agreement No. 0335765 , National Nanotechnology Infrastructure Network, with Cornell University.
PY - 2012/4/15
Y1 - 2012/4/15
N2 - Several recent studies have demonstrated that zwitterionic ultrafiltration (UF) membranes show very low fouling behavior due to the highly hydrated structure of the zwitterions. The objective of this study was to examine the rate of protein transport through a series of semipermeable zwitterionic ultrafiltration membranes generated by chemical modification of a base cellulose membrane. The extent of membrane modification was evaluated by X-ray photoelectron spectroscopy with the effective membrane surface charge density evaluated using streaming potential measurements. Ultrafiltration experiments were performed with basic, neutral, and acidic proteins (cytochrome c, myoglobin, and α-lactalbumin). Protein transport through the zwitterionic membranes was a strong function of solution conditions due to the electrostatic interactions between the charged proteins and the zwitterionic ligands. The protein sieving coefficient was well correlated with the product of the surface charge densities of the protein and membrane. There was no evidence of membrane fouling, even under conditions where the protein and membrane had opposite polarity. The results demonstrate the potential of using zwitterionic ligands to generate high performance ultrafiltration membranes for bioprocessing applications.
AB - Several recent studies have demonstrated that zwitterionic ultrafiltration (UF) membranes show very low fouling behavior due to the highly hydrated structure of the zwitterions. The objective of this study was to examine the rate of protein transport through a series of semipermeable zwitterionic ultrafiltration membranes generated by chemical modification of a base cellulose membrane. The extent of membrane modification was evaluated by X-ray photoelectron spectroscopy with the effective membrane surface charge density evaluated using streaming potential measurements. Ultrafiltration experiments were performed with basic, neutral, and acidic proteins (cytochrome c, myoglobin, and α-lactalbumin). Protein transport through the zwitterionic membranes was a strong function of solution conditions due to the electrostatic interactions between the charged proteins and the zwitterionic ligands. The protein sieving coefficient was well correlated with the product of the surface charge densities of the protein and membrane. There was no evidence of membrane fouling, even under conditions where the protein and membrane had opposite polarity. The results demonstrate the potential of using zwitterionic ligands to generate high performance ultrafiltration membranes for bioprocessing applications.
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U2 - 10.1016/j.memsci.2011.12.021
DO - 10.1016/j.memsci.2011.12.021
M3 - Article
AN - SCOPUS:84857500478
SN - 0376-7388
VL - 397-398
SP - 1
EP - 8
JO - Journal of Membrane Science
JF - Journal of Membrane Science
ER -