Abstract
We used a proteomics approach (iTRAQ) to identify differentially expressed lung proteins during early stages of the tobacco carcinogen, NNK-induced lung tumorigenesis (hyperplasia/atypia, 4-10 weeks and adenoma, 25 week) prior to the detection of adenocarcinoma in A/J mice. We identified 790 proteins at week 4 and 710 proteins at weeks 10 and 25 with >95% confidence and a Local False Discovery Rate estimation less than 5%. We have demonstrated that NNK altered multiple protein (e.g., Carbonyl Reductase, Annexin A5/6, Selenbp1, Hmgb1 and Hsp90b1) expressions in early lesions which can be used as potential biomarkers for early detection of lung cancer.
Original language | English (US) |
---|---|
Pages (from-to) | 23-33 |
Number of pages | 11 |
Journal | EuPA Open Proteomics |
Volume | 9 |
DOIs | |
State | Published - Dec 1 2015 |
All Science Journal Classification (ASJC) codes
- Biochemistry