Proteomic profiling of hyperplasia/atypia and adenoma-induced by NNK in mouse lung identified multiple proteins as potential biomarkers for early detection

Arunangshu Das; James D. Bortner; Cesar Aliaga; Timothy Cooper; Anne Stanley; Bruce A. Stanley; Chandra P. Belani; Karam El-Bayoumy

doi:10.1016/j.euprot.2015.07.015

Proteomic profiling of hyperplasia/atypia and adenoma-induced by NNK in mouse lung identified multiple proteins as potential biomarkers for early detection

Arunangshu Das, James D. Bortner, Cesar Aliaga, Timothy Cooper, Anne Stanley, Bruce A. Stanley, Chandra P. Belani, Karam El-Bayoumy

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Abstract

We used a proteomics approach (iTRAQ) to identify differentially expressed lung proteins during early stages of the tobacco carcinogen, NNK-induced lung tumorigenesis (hyperplasia/atypia, 4-10 weeks and adenoma, 25 week) prior to the detection of adenocarcinoma in A/J mice. We identified 790 proteins at week 4 and 710 proteins at weeks 10 and 25 with >95% confidence and a Local False Discovery Rate estimation less than 5%. We have demonstrated that NNK altered multiple protein (e.g., Carbonyl Reductase, Annexin A5/6, Selenbp1, Hmgb1 and Hsp90b1) expressions in early lesions which can be used as potential biomarkers for early detection of lung cancer.

Original language	English (US)
Pages (from-to)	23-33
Number of pages	11
Journal	EuPA Open Proteomics
Volume	9
DOIs	https://doi.org/10.1016/j.euprot.2015.07.015
State	Published - Dec 1 2015

All Science Journal Classification (ASJC) codes

Biochemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.euprot.2015.07.015

Cite this

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title = "Proteomic profiling of hyperplasia/atypia and adenoma-induced by NNK in mouse lung identified multiple proteins as potential biomarkers for early detection",

abstract = "We used a proteomics approach (iTRAQ) to identify differentially expressed lung proteins during early stages of the tobacco carcinogen, NNK-induced lung tumorigenesis (hyperplasia/atypia, 4-10 weeks and adenoma, 25 week) prior to the detection of adenocarcinoma in A/J mice. We identified 790 proteins at week 4 and 710 proteins at weeks 10 and 25 with >95% confidence and a Local False Discovery Rate estimation less than 5%. We have demonstrated that NNK altered multiple protein (e.g., Carbonyl Reductase, Annexin A5/6, Selenbp1, Hmgb1 and Hsp90b1) expressions in early lesions which can be used as potential biomarkers for early detection of lung cancer.",

author = "Arunangshu Das and Bortner, {James D.} and Cesar Aliaga and Timothy Cooper and Anne Stanley and Stanley, {Bruce A.} and Belani, {Chandra P.} and Karam El-Bayoumy",

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doi = "10.1016/j.euprot.2015.07.015",

language = "English (US)",

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T1 - Proteomic profiling of hyperplasia/atypia and adenoma-induced by NNK in mouse lung identified multiple proteins as potential biomarkers for early detection

AU - Das, Arunangshu

AU - Bortner, James D.

AU - Aliaga, Cesar

AU - Cooper, Timothy

AU - Stanley, Anne

AU - Stanley, Bruce A.

AU - Belani, Chandra P.

AU - El-Bayoumy, Karam

PY - 2015/12/1

Y1 - 2015/12/1

N2 - We used a proteomics approach (iTRAQ) to identify differentially expressed lung proteins during early stages of the tobacco carcinogen, NNK-induced lung tumorigenesis (hyperplasia/atypia, 4-10 weeks and adenoma, 25 week) prior to the detection of adenocarcinoma in A/J mice. We identified 790 proteins at week 4 and 710 proteins at weeks 10 and 25 with >95% confidence and a Local False Discovery Rate estimation less than 5%. We have demonstrated that NNK altered multiple protein (e.g., Carbonyl Reductase, Annexin A5/6, Selenbp1, Hmgb1 and Hsp90b1) expressions in early lesions which can be used as potential biomarkers for early detection of lung cancer.

AB - We used a proteomics approach (iTRAQ) to identify differentially expressed lung proteins during early stages of the tobacco carcinogen, NNK-induced lung tumorigenesis (hyperplasia/atypia, 4-10 weeks and adenoma, 25 week) prior to the detection of adenocarcinoma in A/J mice. We identified 790 proteins at week 4 and 710 proteins at weeks 10 and 25 with >95% confidence and a Local False Discovery Rate estimation less than 5%. We have demonstrated that NNK altered multiple protein (e.g., Carbonyl Reductase, Annexin A5/6, Selenbp1, Hmgb1 and Hsp90b1) expressions in early lesions which can be used as potential biomarkers for early detection of lung cancer.

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Proteomic profiling of hyperplasia/atypia and adenoma-induced by NNK in mouse lung identified multiple proteins as potential biomarkers for early detection

Abstract

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