Protocol to identify and sort antigen-specific B cells from Plasmodium-infected mice

Carolina Calôba; Allen M. Minns; Scott E. Lindner; Rahul Vijay

doi:10.1016/j.xpro.2025.104129

Protocol to identify and sort antigen-specific B cells from Plasmodium-infected mice

Carolina Calôba
, Allen M. Minns
, Scott E. Lindner
, Rahul Vijay

Research output: Contribution to journal › Article › peer-review

Abstract

Here, we detail a protocol to identify an antigen-specific B cell population using SpyCage reagents tailored to bind MSP_1-19 antigen in a rodent malaria model. We describe steps for the preparation of splenic single-cell suspension, B cell enrichment, and staining to facilitate sorting of live cells for downstream applications such as single-cell RNA, V(D)J, and assay for transposase-accessible chromatin (ATAC) sequencing. For complete details on the use and execution of this protocol, please refer to Calôba et al.¹

Original language	English (US)
Article number	104129
Journal	STAR Protocols
Volume	6
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2025.104129
State	Published - Dec 19 2025

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

General Neuroscience
General Immunology and Microbiology
General Biochemistry, Genetics and Molecular Biology

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10.1016/j.xpro.2025.104129

Cite this

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title = "Protocol to identify and sort antigen-specific B cells from Plasmodium-infected mice",

abstract = "Here, we detail a protocol to identify an antigen-specific B cell population using SpyCage reagents tailored to bind MSP1-19 antigen in a rodent malaria model. We describe steps for the preparation of splenic single-cell suspension, B cell enrichment, and staining to facilitate sorting of live cells for downstream applications such as single-cell RNA, V(D)J, and assay for transposase-accessible chromatin (ATAC) sequencing. For complete details on the use and execution of this protocol, please refer to Cal{\^o}ba et al.1",

author = "Carolina Cal{\^o}ba and Minns, \{Allen M.\} and Lindner, \{Scott E.\} and Rahul Vijay",

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doi = "10.1016/j.xpro.2025.104129",

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AU - Calôba, Carolina

AU - Minns, Allen M.

AU - Lindner, Scott E.

AU - Vijay, Rahul

PY - 2025/12/19

Y1 - 2025/12/19

N2 - Here, we detail a protocol to identify an antigen-specific B cell population using SpyCage reagents tailored to bind MSP1-19 antigen in a rodent malaria model. We describe steps for the preparation of splenic single-cell suspension, B cell enrichment, and staining to facilitate sorting of live cells for downstream applications such as single-cell RNA, V(D)J, and assay for transposase-accessible chromatin (ATAC) sequencing. For complete details on the use and execution of this protocol, please refer to Calôba et al.1

AB - Here, we detail a protocol to identify an antigen-specific B cell population using SpyCage reagents tailored to bind MSP1-19 antigen in a rodent malaria model. We describe steps for the preparation of splenic single-cell suspension, B cell enrichment, and staining to facilitate sorting of live cells for downstream applications such as single-cell RNA, V(D)J, and assay for transposase-accessible chromatin (ATAC) sequencing. For complete details on the use and execution of this protocol, please refer to Calôba et al.1

UR - https://www.scopus.com/pages/publications/105018633369

UR - https://www.scopus.com/pages/publications/105018633369#tab=citedBy

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DO - 10.1016/j.xpro.2025.104129

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AN - SCOPUS:105018633369

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VL - 6

JO - STAR Protocols

JF - STAR Protocols

IS - 4

M1 - 104129

ER -

Protocol to identify and sort antigen-specific B cells from Plasmodium-infected mice

Abstract

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All Science Journal Classification (ASJC) codes

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