@article{300b120e32034c69888c4e3808982cc0,
title = "PsaE- and NdhF-mediated electron transport affect bicarbonate transport rather than carbon dioxide uptake in the cyanobacterium Synechococcus sp. PCC7002",
abstract = "The wild-type and three deletional interposon mutants (psaE, ndhF, psaE/ndhF) of Synechococcus sp. PCC7002 were analysed with respect to their ability to induce the CO2-concentrating mechanism (CCM). The response of the ndhF mutant was similar to that of the control with normal adaptation of CO2 and HCO3/- transport at low CO2. In contrast, the psaE mutant was unable to grow normally at 20 μL·L-1 and could not induce high-affinity HCO3/- uptake. The double mutant showed an even greater degree of impaired growth than the psaE mutant, even at 350 μl·L-1 CO2. It also showed a relatively specific defect in the ability of the cells to induce high- affinity HCO3/--uptake, although when growth was severely restricted at 20 μL.L-1 CO2 there may also have been some effect on the induction of CO2 transport. Our results indicate a key role for psaE-mediated, and to a lesser extent ndhF-mediated, electron transport in efficient utilisation of HCO3/- under low inorganic carbon in Synechococcus sp. PCC7002.",
author = "Dieter S{\"u}ltemeyer and Price, {G. Dean} and Bryant, {Donald A.} and Badger, {Murray R.}",
note = "Funding Information: Fig. 5A-B. The modelled response of photosynthesis (A) and internal Ci (B) to external Ci in high-and low-Ci ceils of Synechococcus PCC7002. The data were calculated according to Badger et al. 1991. The following cell parameters were assumed: external pH 8.0; cytosolic pH, 7.6; carboxysome pH, 7.6; plasmalemma CO2 and HCO~-conductances, 10 2 and 10 .7 cm. s-l; carboxysome CO2 and HCO;-conductances, 10 .5 and 10-4cms 1; Vr~,x Rubisco, 5.2×10-I2gmol-cell-1{"}s-1; Ko.s (CO2) Rubiscoo 0.15mM; cytosolic cell volume, 1.89 x 10 12 cm 3,cell-I; carboxysome cell volume, 4.2 x 10 14 cm 3 {"} cell-1; plasmalemma area, 7.3x10 8cm 2.cell-I; carboxysome shell area, 1.27x10-8 cm 2. cell-1; no cytosolic carbonic anhydrase and x 10 000 catalysis in the carboxysome; Km (Ci) for Ci transport, 0.01 and 0.15 mM for high-and low-Ci cells, respectively; Vm~ for Ci transport, 6.3 x 10-lz and 5.6 × 10-~2 gmol. cell-t. s l for high-and low-Ci cells D.S. is a recipient of a research fellowship from the Deutsche Forschungsgemeinschaft. Part of the research was also supported by grant DMB-9206851 from the National Science Foundation to D.A.B.",
year = "1997",
month = jan,
doi = "10.1007/bf01258678",
language = "English (US)",
volume = "201",
pages = "36--42",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "1",
}