PU.1 expression delineates heterogeneity in primary Th2 cells

Hua Chen Chang; Shangming Zhang; Vivian T. Thieu; Roger B. Slee; Heather A. Bruns; R. Nicholas Laribee; Michael J. Klemsz; Mark H. Kaplan

doi:10.1016/j.immuni.2005.03.016

PU.1 expression delineates heterogeneity in primary Th2 cells

Hua Chen Chang, Shangming Zhang, Vivian T. Thieu, Roger B. Slee, Heather A. Bruns, R. Nicholas Laribee, Michael J. Klemsz, Mark H. Kaplan

Department of Physical Medicine and Rehabilitation

Research output: Contribution to journal › Article › peer-review

133 Scopus citations

Abstract

Primary T helper 2 cells are heterogeneous, expressing subsets of cytokines at varying levels. Mechanisms controlling this spectrum of phenotypes are still unclear. The ETS family transcription factor PU.1 is expressed in Th2 but not Th1 cells. Th2 cytokine production is decreased in cultures transduced with a PU.1-expressing retrovirus and increased in Th2 cells following RNAi that decreases PU.1 expression. In primary cultures, PU.1 expression is restricted to a subpopulation of Th2 cells that express CCL22 and a subset of Th2 cytokines. PU.1 regulates the Th2 phenotype by interfering with GATA-3 DNA binding without altering GATA-3 protein levels. Thus, the expression of PU.1 in subsets of Th2 cells establishes a defined cytokine profile and contributes towards establishing the spectrum of cytokine production observed in Th2 populations.

Original language	English (US)
Pages (from-to)	693-703
Number of pages	11
Journal	Immunity
Volume	22
Issue number	6
DOIs	https://doi.org/10.1016/j.immuni.2005.03.016
State	Published - Jun 2005

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Infectious Diseases

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10.1016/j.immuni.2005.03.016

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@article{9b584fdc4fb240699dc96c840e916772,

title = "PU.1 expression delineates heterogeneity in primary Th2 cells",

abstract = "Primary T helper 2 cells are heterogeneous, expressing subsets of cytokines at varying levels. Mechanisms controlling this spectrum of phenotypes are still unclear. The ETS family transcription factor PU.1 is expressed in Th2 but not Th1 cells. Th2 cytokine production is decreased in cultures transduced with a PU.1-expressing retrovirus and increased in Th2 cells following RNAi that decreases PU.1 expression. In primary cultures, PU.1 expression is restricted to a subpopulation of Th2 cells that express CCL22 and a subset of Th2 cytokines. PU.1 regulates the Th2 phenotype by interfering with GATA-3 DNA binding without altering GATA-3 protein levels. Thus, the expression of PU.1 in subsets of Th2 cells establishes a defined cytokine profile and contributes towards establishing the spectrum of cytokine production observed in Th2 populations.",

author = "Chang, {Hua Chen} and Shangming Zhang and Thieu, {Vivian T.} and Slee, {Roger B.} and Bruns, {Heather A.} and Laribee, {R. Nicholas} and Klemsz, {Michael J.} and Kaplan, {Mark H.}",

note = "Funding Information: We thank Drs. A. Dent, C.-H. Chang, J. Blum and G. Kansas for critical review of this manuscript, Dr. Harinder Singh for graciously providing PU.1-heterozygous mice, Nick Lukacs for performing the CCL22 ELISA, and the staff of the Indiana University Cancer Center Flow Cytometry Facility. This work was supported by Public Health Service Awards AI45515 and AI57459 (MHK), training grants DK007519 (HCC) and HL007910 (VTT), and the Indiana Genomics Initiative of Indiana University, which is supported in part by the Lilly endowment.",

year = "2005",

month = jun,

doi = "10.1016/j.immuni.2005.03.016",

language = "English (US)",

volume = "22",

pages = "693--703",

journal = "Immunity",

issn = "1074-7613",

publisher = "Cell Press",

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AU - Chang, Hua Chen

AU - Zhang, Shangming

AU - Thieu, Vivian T.

AU - Slee, Roger B.

AU - Bruns, Heather A.

AU - Laribee, R. Nicholas

AU - Klemsz, Michael J.

AU - Kaplan, Mark H.

N1 - Funding Information: We thank Drs. A. Dent, C.-H. Chang, J. Blum and G. Kansas for critical review of this manuscript, Dr. Harinder Singh for graciously providing PU.1-heterozygous mice, Nick Lukacs for performing the CCL22 ELISA, and the staff of the Indiana University Cancer Center Flow Cytometry Facility. This work was supported by Public Health Service Awards AI45515 and AI57459 (MHK), training grants DK007519 (HCC) and HL007910 (VTT), and the Indiana Genomics Initiative of Indiana University, which is supported in part by the Lilly endowment.

PY - 2005/6

Y1 - 2005/6

N2 - Primary T helper 2 cells are heterogeneous, expressing subsets of cytokines at varying levels. Mechanisms controlling this spectrum of phenotypes are still unclear. The ETS family transcription factor PU.1 is expressed in Th2 but not Th1 cells. Th2 cytokine production is decreased in cultures transduced with a PU.1-expressing retrovirus and increased in Th2 cells following RNAi that decreases PU.1 expression. In primary cultures, PU.1 expression is restricted to a subpopulation of Th2 cells that express CCL22 and a subset of Th2 cytokines. PU.1 regulates the Th2 phenotype by interfering with GATA-3 DNA binding without altering GATA-3 protein levels. Thus, the expression of PU.1 in subsets of Th2 cells establishes a defined cytokine profile and contributes towards establishing the spectrum of cytokine production observed in Th2 populations.

AB - Primary T helper 2 cells are heterogeneous, expressing subsets of cytokines at varying levels. Mechanisms controlling this spectrum of phenotypes are still unclear. The ETS family transcription factor PU.1 is expressed in Th2 but not Th1 cells. Th2 cytokine production is decreased in cultures transduced with a PU.1-expressing retrovirus and increased in Th2 cells following RNAi that decreases PU.1 expression. In primary cultures, PU.1 expression is restricted to a subpopulation of Th2 cells that express CCL22 and a subset of Th2 cytokines. PU.1 regulates the Th2 phenotype by interfering with GATA-3 DNA binding without altering GATA-3 protein levels. Thus, the expression of PU.1 in subsets of Th2 cells establishes a defined cytokine profile and contributes towards establishing the spectrum of cytokine production observed in Th2 populations.

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PU.1 expression delineates heterogeneity in primary Th2 cells

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