TY - JOUR
T1 - Pulsed Focused Ultrasound Treatment of Muscle Mitigates Paralysis-Induced Bone Loss in the Adjacent Bone
T2 - A Study in a Mouse Model
AU - Poliachik, Sandra L.
AU - Khokhlova, Tatiana D.
AU - Wang, Yak Nam
AU - Simon, Julianna C.
AU - Bailey, Michael R.
N1 - Funding Information:
This work was supported, in part, by DoD Award SC090510 and the National Space Biomedical Research Institute through NCC 9-58. The authors thank Ted S. Gross, Department of Orthopaedics, University of Washington, for his generous support of the pilot project that launched this work.
PY - 2014/9
Y1 - 2014/9
N2 - Bone loss can result from bed rest, space flight, spinal cord injury or age-related hormonal changes. Current bone loss mitigation techniques include pharmaceutical interventions, exercise, pulsed ultrasound targeted to bone and whole body vibration. In this study, we attempted to mitigate paralysis-induced bone loss by applying focused ultrasound to the midbelly of a paralyzed muscle. We employed a mouse model of disuse that uses onabotulinumtoxinA-induced paralysis, which causes rapid bone loss in 5 d. A focused 2 MHz transducer applied pulsed exposures with pulse repetition frequency mimicking that of motor neuron firing during walking (80 Hz), standing (20 Hz), or the standard pulsed ultrasound frequency used in fracture healing (1 kHz). Exposures were applied daily to calf muscle for 4 consecutive d. Trabecular bone changes were characterized using micro-computed tomography. Our results indicated that application of certain focused pulsed ultrasound parameters was able to mitigate some of the paralysis-induced bone loss.
AB - Bone loss can result from bed rest, space flight, spinal cord injury or age-related hormonal changes. Current bone loss mitigation techniques include pharmaceutical interventions, exercise, pulsed ultrasound targeted to bone and whole body vibration. In this study, we attempted to mitigate paralysis-induced bone loss by applying focused ultrasound to the midbelly of a paralyzed muscle. We employed a mouse model of disuse that uses onabotulinumtoxinA-induced paralysis, which causes rapid bone loss in 5 d. A focused 2 MHz transducer applied pulsed exposures with pulse repetition frequency mimicking that of motor neuron firing during walking (80 Hz), standing (20 Hz), or the standard pulsed ultrasound frequency used in fracture healing (1 kHz). Exposures were applied daily to calf muscle for 4 consecutive d. Trabecular bone changes were characterized using micro-computed tomography. Our results indicated that application of certain focused pulsed ultrasound parameters was able to mitigate some of the paralysis-induced bone loss.
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U2 - 10.1016/j.ultrasmedbio.2014.02.027
DO - 10.1016/j.ultrasmedbio.2014.02.027
M3 - Article
C2 - 24857416
AN - SCOPUS:84905589103
SN - 0301-5629
VL - 40
SP - 2113
EP - 2124
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
IS - 9
ER -