Purification of glycineamide ribonucleotide transformylase

C. A. Caperelli; G. Chettur; L. Y. Lin; S. J. Benkovic

doi:10.1016/0006-291X(78)90890-2

Purification of glycineamide ribonucleotide transformylase

C. A. Caperelli, G. Chettur, L. Y. Lin, S. J. Benkovic

Chemistry

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17 Scopus citations

Abstract

Glycineamide ribonucleotide transformylase has been purified 240-fold from chicken liver along with formyl-methenyl-methylenetetrahydrofolate synthetase (combined), through seven purification steps including a glycineamide ribonucleotide affinity column and a 10-formyl synthetase specific MgATP elution from hydroxylapatite. The transformylase is a homodimer of M_r 63,000 whereas the synthetase (combined) is composed of two identical subunits of M_r 83,000. Transformylase has been isolated free of the synthetase (combined) by an additional anion exchange chromatographic step resulting in a net 400-fold purification.

Original language	English (US)
Pages (from-to)	403-410
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	82
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(78)90890-2
State	Published - May 30 1978

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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AU - Caperelli, C. A.

AU - Chettur, G.

AU - Lin, L. Y.

AU - Benkovic, S. J.

PY - 1978/5/30

Y1 - 1978/5/30

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AB - Glycineamide ribonucleotide transformylase has been purified 240-fold from chicken liver along with formyl-methenyl-methylenetetrahydrofolate synthetase (combined), through seven purification steps including a glycineamide ribonucleotide affinity column and a 10-formyl synthetase specific MgATP elution from hydroxylapatite. The transformylase is a homodimer of Mr 63,000 whereas the synthetase (combined) is composed of two identical subunits of Mr 83,000. Transformylase has been isolated free of the synthetase (combined) by an additional anion exchange chromatographic step resulting in a net 400-fold purification.

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Purification of glycineamide ribonucleotide transformylase

Abstract

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