TY - JOUR
T1 - Purinergic receptor expression and function in rat vagal sensory neurons innervating the stomach
AU - Blanke, Emily N.
AU - Stella, Salvatore L.
AU - Ruiz-Velasco, Victor
AU - Holmes, Gregory M.
N1 - Funding Information:
This work was supported by National Institutes of Health Grant NINDS 49177 (G.M.H.)and funded in part under a grant with the Pennsylvania Department of Health using Tobacco CURE funds (S.L.S.). The authors would like to acknowledge the technical and general assistance of Lisa B. Willing.
Funding Information:
This work was supported by National Institutes of Health Grant NINDS 49177 (G.M.H.) and funded in part under a grant with the Pennsylvania Department of Health using Tobacco CURE funds (S.L.S.).
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/7/27
Y1 - 2019/7/27
N2 - The nodose ganglion (NG)is the main parasympathetic ganglion conveying sensory signals to the CNS from numerous visceral organs including digestive signals such as gastric distension or the release the gastrointestinal peptides. The response characteristics of NG neurons to ATP and ADP and pharmacological interrogation of purinergic receptor subtypes have been previously investigated but often in NG cells of undetermined visceral origin. In this study, we confirmed the presence of P2X3 and P2Y1 receptors and characterized P2X and P2Y responses in gastric-innervating NG neurons. Application of ATP-evoked large inward currents and cytosolic Ca2+ increases in gastric-innervating NG neurons. Despite the expression of P2Y1 receptors, ADP elicited only minor modulation of voltage-gated Ca2+ channels. Considering the sensitivity of NG neurons to comorbidities associated with disease or neural injury, purinergic modulation of gastric NG neurons in disease- or injury-states is worthy of further investigation.
AB - The nodose ganglion (NG)is the main parasympathetic ganglion conveying sensory signals to the CNS from numerous visceral organs including digestive signals such as gastric distension or the release the gastrointestinal peptides. The response characteristics of NG neurons to ATP and ADP and pharmacological interrogation of purinergic receptor subtypes have been previously investigated but often in NG cells of undetermined visceral origin. In this study, we confirmed the presence of P2X3 and P2Y1 receptors and characterized P2X and P2Y responses in gastric-innervating NG neurons. Application of ATP-evoked large inward currents and cytosolic Ca2+ increases in gastric-innervating NG neurons. Despite the expression of P2Y1 receptors, ADP elicited only minor modulation of voltage-gated Ca2+ channels. Considering the sensitivity of NG neurons to comorbidities associated with disease or neural injury, purinergic modulation of gastric NG neurons in disease- or injury-states is worthy of further investigation.
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U2 - 10.1016/j.neulet.2019.05.017
DO - 10.1016/j.neulet.2019.05.017
M3 - Article
C2 - 31085293
AN - SCOPUS:85066069373
SN - 0304-3940
VL - 706
SP - 182
EP - 188
JO - Neuroscience letters
JF - Neuroscience letters
ER -